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==Science in the city== | ==Science in the city== | ||
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''This is a series of high content essays about the every day life of scientists in berlin. If you really have the time, or your boss is not around at the moment, then read ahead and enjoy.'' | ''This is a series of high content essays about the every day life of scientists in berlin. If you really have the time, or your boss is not around at the moment, then read ahead and enjoy.'' | ||
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'''DAY 1''' {{hide | | '''DAY 1''' {{hide | | ||
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They took this nice fotograph of me<br> | They took this nice fotograph of me<br> | ||
in the case anybody would be missing me.<br> | in the case anybody would be missing me.<br> | ||
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Revision as of 04:00, 15 March 2009
I am a new member of OpenWetWare!
Contact Info
- Victor Tapia
- Charité - Medical School
- hessischer Str. 3-4
- 10115 Berlin
- Berlin, Germany
- Email me through OpenWetWare
I work in the Inst. f. Med. Immunol. at the Charité Medical School of Berlin in the research group of Dr. R. Volkmer [1,2].
Education
- 2009?, PhD, Institute for Medical Immunology, Charité Medical School, Berlin
- 2004, MS, Institute of Biology, Humboldt University, Berlin
- 2002, BS, Institute of Biology, Humboldt University, Berlin
Research interests
- Modularity of Protein Structure and Cellular Signal Transduction.
- Protein Folding and Neurodegenerative Deseases.
- Natural Autoimmunology and Diagnostic Assays
Publications
-
Tapia & Volkmer (2009) "Exploring and profiling protein function with peptide arrays" in: Methods in molecular biology (eds. Chiari). Humana Press
This work reviews the peptide array technology focusing on technological advancements, oriented immobilization of peptide probes, and some interesting applications. Going along the philosophy of the monography series, we give some general recomendations on methods and instruments.
- Tapia V, Ay B, Triebus J, Wolter E, Boisguerin P, and Volkmer R. Evaluating the coupling efficiency of phosphorylated amino acids for SPOT synthesis. J Pept Sci. 2008 Dec;14(12):1309-14. DOI:10.1002/psc.1068 |
This work was carried out to adapt "building block" approaches for the generation of phosphopeptides through resine-supported solid-phase peptide synthesis to the spot-technology, which relies on cellulose as support. It compares different coupling strategies for the incorporation of protected phoshoaminiacids into determined positions of synthetic peptides. We find that the use of EEDQ (caution!, neurotoxic) as coupling activator is the most efficient strategy in our comparison.
- Tapia V, Bongartz J, Schutkowski M, Bruni N, Weiser A, Ay B, Volkmer R, and Or-Guil M. Affinity profiling using the peptide microarray technology: a case study. Anal Biochem. 2007 Apr 1;363(1):108-18. DOI:10.1016/j.ab.2006.12.043 |
This work analyses the predictive potential of microarray-based binding assays. Fluorescent SI-measurements were used to fit a mass-action derived model estimating the observed affinity (equil. diss. const.).
- Weiser AA, Or-Guil M, Tapia V, Leichsenring A, Schuchhardt J, Frömmel C, and Volkmer-Engert R. SPOT synthesis: reliability of array-based measurement of peptide binding affinity. Anal Biochem. 2005 Jul 15;342(2):300-11. DOI:10.1016/j.ab.2005.04.033 |
This work analyses the quantitative potential of measurements derived from peptide macroarray-based immunobinding assays. Chemoluminiscent signal intensity data was confronted with independent "golden stardard" measurements (surface plasmon resonance-based binding assays on a BIAcoreX maschine).
Presentations
- V. Tapia & R. Volkmer; Steady State Analysis of Peptide Array-based Binding Assays: A Mathematical Model for Disease Diagnostic and Affinity Profiling of Bimolecular Interactions. 9th German Peptide Symposium, 2009, Göttingen FIGURE
Useful links
- http://openwetware.org/wiki/Molecular_Recognition_Laboratorium
- http://openwetware.org/wiki/Template:Molecular_Recognition
- http://openwetware.org/wiki/User:Victor_Tapia/Notebook/pqbp1
- http://immunologie.charite.de/arbeitsgruppen/ag-volkmer/
Science in the city
This is a series of high content essays about the every day life of scientists in berlin. If you really have the time, or your boss is not around at the moment, then read ahead and enjoy.
DAY 1
i was just returning from an international symposium
and, naturally, the first thing i did once in berlin
was to go to the lab and get some very intersting things done.
It was not necesary to do it right away,
but my sense of responsability was taking over me.
At the moment, i did not know that this desition
was about to change my whole carreer.
But i only made it to the stairs
because a bunch of funny guys
intercepted me on the way.
They were all very disappointed
about the attitude of scientists
saying: "these people are always thinking
about cheap prices and bonus".
They all belong to the provider-rebel-army
and wanted me to go with them on a special vacation.
The guys were quite squared headed, so i complied.
They have been treating me very nicely, since then
and have total freedom of speech, as you can see
They took this nice fotograph of me
in the case anybody would be missing me.
