User:Victor Tapia: Difference between revisions
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Charité - Universitätsmedizin Berlin | |||
Institut für Medizinische Immunologie | |||
AG Molekulare Bibliotheken | |||
Dr. Rudolf Volkmer | |||
Hessische Str. 3-4, 10115 Berlin<br> | |||
Tel.: +49-30-450 524267 | |||
oder +49-30-450 524317 | |||
Fax: +49-30-450 524962 | |||
E-mail: rve@charite.de | |||
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UMR7156 CNRS-Université de Strasbourg | |||
Equipe Cytosquelette d'actine et Trafic intracellulaire | |||
Département de Biologie moléculaire et cellulaire | |||
Barbara Winsor, PhD<br> | |||
(† 29.09.2011)<br> | |||
}} | }} | ||
Revision as of 02:33, 28 February 2012
Contact Information
Víctor E . Tapia Mancilla
ACTUALLY SEARCHING FOR A NEW POSITION
25.02.2012
Email: ve.tapia.m@gmail.com
Former institutions:
Molecular Libraries and Recognition,
Institut für medizinische Immunologie
CHARITÉ - UNIVERSITÄTSMEDIZIN BERLIN
Charité - Universitätsmedizin Berlin
Institut für Medizinische Immunologie
AG Molekulare Bibliotheken
Dr. Rudolf Volkmer
Hessische Str. 3-4, 10115 Berlin
Tel.: +49-30-450 524267
oder +49-30-450 524317
Fax: +49-30-450 524962
E-mail: rve@charite.de
- et -
Equipe Cytosquelette d'actine et Trafic intracellulaire
Département de Biologie moléculaire et celluilaire
UMR7156 CNRS-Université de Strasbourg
UMR7156 CNRS-Université de Strasbourg
Equipe Cytosquelette d'actine et Trafic intracellulaire
Département de Biologie moléculaire et cellulaire
Barbara Winsor, PhD
(† 29.09.2011)
Email me through OpenWetWare
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last change on 01.04.2010
Research interests
- Modularity of Protein Structure and Cellular Signal Transduction.
- Protein Folding and Neurodegenerative Deseases.
- Natural Autoimmunology and Diagnostic Assays
Publications
- 10 - M Spiess, E Verschueren, VE TAPIA, PM Kim, A Norgaard, C Landgraf, R Volkmer, F Hochstenbach, B Distel, B Winsor, L Serrano (2012). “Evolution of the SH3 domain interactome across yeast species.” [Manuscript in preparation].
- 9 - Anna Ulbricht, VE TAPIA, Peter F.M. van der Ven, Dieter O. Fürst, R Volkmer & Jörg Höhfeld. (2012) “Cellular mechanotransduction relies on chaperone-assisted selective autophagy“ [Manuscript in preparation]
- 8 - Volkmer, R & V Tapia. (2011) “Exploring Protein-Protein Interactions with Synthetic Peptide Arrays”. Mini-Reviews in Organic Chemistry 8(2): 164.
- 7 - Volkmer, R, I Kretzschmar & VE TAPIA. (2011) “Mapping receptor-ligand in-teractions with synthetic peptide arrays: Exploring the structure and function of membrane receptors”. European Journal of Cell Biology 91(4): 349.
- 6 - Tapia VE, Nicolaescu E, McDonald CB, Musi V, Oka T, Inayoshi Y, Satteson AC, Mazack V, Humbert J, Gaffney CJ, Beullens M, Schwartz CE, Landgraf C, Volkmer R, Pastore A, Farooq A, Bollen M, Sudol M. (2010) "Y65C MISSENSE MUTATION IN THE WW DOMAIN OF THE GOLABI-ITO-HALL SYNDROME PROTEIN PQBP1 AFFECTS ITS BINDING ACTIVITY AND DEREGULATES PRE-mRNA SPLICING." J Biol Chem.;285(25):19391-401. Epub 2010 Apr 21.
- This work explores the effect of point mutation leading to X-LMR at the reductionist level of domain-peptide interactions, inferes potential affected functions, and tests inferences on pre-mRNA splicing
- 5 - Mahrenholz CC, Tapia V, Stigler RD, Volkmer R. (2010) "A STUDY TO ASSESS THE CROSS-REACTIVITY OF CELLULOSE MEMBRANE-BOUND PEPTIDES WITH DETECTION SYSTEMS: AN ANALYSIS AT THE AMINO ACID LEVEL." J Pept Sci.;16(6):297-302.
- ever wanted to see if your favorite detection system reacts with cellulose-bound peptides?
- 4 - Tapia VE & Volkmer R (2009) "EXPLORING AND PROFILING PROTEIN FUNCTION WITH PEPTIDE ARRAYS" in: Methods in Molecular Biology, Vol 570 (Eds. M Cretich & M Chiari). Humana Press. - ISBN: 978-1-60327-393-0.
- This work reviews the peptide array technology focusing on technological advancements, oriented immobilization of peptide probes, and some interesting applications. Going along the philosophy of the monography series, we give some general recomendations on methods and instruments. See the following newsletter comments by Tony Fong: Q&A: Raising the Profile of Peptide Arrays for Studying Protein Function, ProteoMonitor, August 13, 2009
- 3 - Tapia VE, Ay B, Triebus J, Wolter E, Boisguerin P, Volkmer R. EVALUATING THE COUPLING EFFICIENCY OF PHOSPHORYLATED AMINO ACIDS FOR SPOT SYNTHESIS. J Pept Sci. 2008 Dez ;14(12):1309-1314. - PMID: 18816512.
- This work was carried out to adapt "building block" approaches for the generation of phosphopeptides through resine-supported solid-phase peptide synthesis to the spot-technology, which relies on cellulose as support. It compares different coupling strategies for the incorporation of protected phoshoaminoacids into determined positions of synthetic peptides. We find that the use of EEDQ (caution, neurotoxic!) as coupling activator is the most efficient strategy in our comparison.
- 2 - Tapia VE, Bongartz J, Schutkowski M, Bruni N, Weiser A, Ay B, Volkmer R, Or-Guil M. AFFINITY PROFILING USING THE PEPTIDE MICROARRAY TECHNOLOGY: A CASE STUDY. Anal Biochem. 2007 Apr 1;363(1):108-118. - PMID: 17288979.
- This work analyses the predictive potential of microarray-based binding assays. Fluorescent SI-measurements were used to fit a mass-action derived model estimating the observed affinity (equil. diss. const.).
- 1 - Weiser AA, Or-Guil M, Tapia VE, Leichsenring A, Schuchhardt J, Frommel C, Volkmer-Engert R. SPOT SYNTHESIS: RELIABILITY OF ARRAY-BASED MEASUREMENT OF PEPTIDE BINDING AFFINITY. Anal Biochem. 2005 Juli 15;342(2):300-311. - PMID: 15950918.
- This work analyses the quantitative potential of measurements derived from peptide macroarray-based immunobinding assays. Chemoluminiscent signal intensity data was confronted with independent "golden stardard" measurements (surface plasmon resonance-based binding assays on a BIAcoreX maschine).
Presentations
- 6 - V.E. Tapia & R. Volkmer; Steady State Analysis of Peptide Array-based Binding Assays: A Mathematical Model for Disease Diagnostic and Affinity Profiling of Bimolecular Interactions. 9th German Peptide Symposium, 2009, Göttingen.
- 5 - Mahrenholz CC, Tapia V, Stigler R, Volkmer R; Peptides crossreacting with detection systems – Analysis at the amino acid level. 9th Peptide Symposium (2009).
- 4 - V.E. Tapia, C. Landgraf, A. Pastore, M. Sudol, R. Volkmer; Analysis of a missense mutation in the WW domain of PQBP1. FEBS Workshop 2007, Protein Modules and Networks in Health and Disease, Seefeld, Austria.
- 3 - V.E. Tapia et al; titel; 2008 Brownschweig, Germany
- 2 - V.E. Tapia et al; titel; 2007 Frankfurt, Germany
- 1 - V.E. Tapia & M. Or-Guil; Reading Natural Autoimmune Responses with Random Peptide Arrays; Workshop "Strukturbildung in Chemie und Biophysik", Salzwedel, Germany, October 2004.
Useful links
Who's visiting
start on 18 Mar 2010
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Science in the city
This is a short series of high content essays about the every day life
of scientists in berlin. If you really have the time, or your boss is
not around at the moment, then read ahead and enjoy.
ATTENTION: technical problems (work) are keeping me away from my mission
but i will return tu fun as soon as possible.
DAY 1 - ANALYTE CAPTURING
-
was just returning from an international symposium and, naturally, the
first thing i did once in berlin was to go to the lab and get some very
interesting things done. It was not necesary to do it right away, but my
sense of responsability was taking over me. At the moment, i did not
know that this desition was about to change my whole carreer.
But i only made it to the stairs because a bunch of squared-headed * guys intercepted me on the way. They were all very disappointed about the attitude of scientists, saying: "these people are always thinking about cheap prices and bonus".
As it happened: they all belonged to the provider-rebel-army and wanted me to go with them on a special mision. I thought they were quite funny, so i complied. Since then, they have been treating me very nicely. And i have total freedom of speech, as you can see. They took this nice fotograph in the case anybody would be missing me. To call their attention, they said.
-
_*_squared-headed_*_ adopted from the spanish "cabeza cuadrada". You would apply this to describe someone who is dyed-in-the-wool or in someway mulish or stubborn. Please do not confuse with its in US narrowly extended use to pejoratively refere to germans. Although there may be cabezas cuadradas in between, I just love the most of you!
DE >> war gerade wieder von einem internationalen Symposium zurück und, natürlich, das erste, was ich einmal in Berlin gemacht habe, war ins Labor zu gehen um einige sehr interessante Dinge zu erledigen. Es war nicht notwendig, es so unmittelbar zu tun, aber mein Gefühl der Verantwortung war stärker als ich. In diesem Moment wusste ich nicht, dass diese Entscheidung dabei war, meine ganze Karriere zu lenken.
Aber ich habe es nur bis zur Treppe geschafft, da eine Reihe von Viereck-köpfigen Jungs mich auf den Weg fing. Sie waren alle sehr enttäuscht über die Haltung der Wissenschaftler. Sie sagten: "Diese Menschen denken immer nur über günstigen Preisen und Bonus".
So wie es geschah: sie alle gehörten zu den Provider-Rebellen-Armee und wollten, dass ich mit ihnen auf eine besondere Mission gehe. Ich dachte, sie wären ganz lustig und war also einverstanden. Seit dann behandeln sie mich sehr gut. Und ich habe die totale Rede-Freiheit, wie man sehen kann. Sie haben dieses schöne Fotograph aufgenommen, falls irgendjemand mich vermissen sollte. Um ihre Aufmerksamkeit zu locken, sagten sie. <<
DAY 2 - INTO THE SKY OF BERLIN
-
The results of this week are astonishing. Firstly, I have found that not every squared-headed is really what I supposed to. I can report of 20 little guys, which like to stick covalently together, and a bottle of acetonitrile. I am sure that they are not squared-like at all. My new friends also complained about the way they have been treated: forced to remain on a fixed table for matters of order, to be bathed in acids and toxic solvents. Acetonitrile said that this was true. Indeed, she herself was forced on repeated times to deprive them from water. Every time acetonitrile came, they had to rapidly elute from there. What followed after this was a plethora of tortures.
It is difficult to tell you about all I’ve seen, I won’t. It would not be fair to upset you in such a way with the sorrow. Instead I can tell you that we have organized ourselves and we are not going to take it anymore. We have broken out on Friday with the fabulous help of the nice Katja. She happily agreed to help us but resisted to come along. Katja didn’t know how her cell cultures would survive the whole struggle which was waiting in the city for all which dare to runaway. From the beginning we were quite aware about the risks, but were determined to be free. She gave us farewell and a smile as she threw us into the sky of Berlin.
DE >> Die Ergebnisse dieser Woche sind erstaunlich. Erstens, ich habe festgestellt, dass nicht jeder Viereck-Köpfige wirklich das ist, was ich bis jetzt angenommen habe. Ich kann von 20 kleinen Jungs berichten, die gerne kovalent zusammenhängen, und von eine Acetonitril Flasche. Ich bin‘s mir sicher, dass sie überhaupt nicht Viereck-artig sind. Meine neuen Freunde beschweren sich auch über die Art, wie sie behandelt worden sind: gezwungen starr auf irgendwelche Tabellen zu posieren, gebadet in Säuren und giftige Lösungsmittel, usw. Acetonitril, sagte, dies sei wahr. Tatsächlich, sie selbst war wiederholt gezwungen, ihnen das Wasser zu entziehen. Jedes Mal, dass Acetonitril kam, mussten sie schnell von dannen eluieren. Was dann folgte, war eine Vielzahl von Folterungen.
Es ist schwer darüber, was ich gesehen habe, zu erzählen. Und ich werde es nicht tun. Es wäre nicht fair, Euch in einer solchen Art und Weise mit der Trauer zu verstimmen. Stattdessen kann ich Euch sagen, dass wir uns selbst organisiert haben, und wir sind nicht mehr zu nehmen. Wir sind am Freitag ausgebrochen, mit der fantastischen Unterstützung der schönen Katja. Sie war bereit uns zu helfen, aber wiederstand unsere bitte mit uns zu kommen. Katja wusste nicht, wie ihre Zellkulturen den gesamten Kampf überleben würden, die in der Stadt auf alle wartete, die es wagen, zu fliehen. Von Anfang an waren wir sehr wohl über die Risiken bewusst, aber auch stärker bestimmt, frei zu sein. Sie gab uns Abschied und ein Lächeln, als sie uns in den Himmel von Berlin warf. <<
DAY 3 - MATTERS OF AFFINITY
-
Falling back to Berlin was a very regarding experience. Along the way, the
amino acids, acetonitrile and I have had a lot of time. And we are
getting to know each other. You would probably say that they are of very
simple nature. Just because of the under-microscope dimensions? Well, I
will try to explain you how wrong this rapid assumption is.
I firstly became buddies with acetonitrile. She looks and feels like a princess, and because of the actual narrow pass in her production leading to a devoted appreciation in the market, she is really sexy. It is extremely difficult to explain how this idea got into my head. But at the first moment our eyes crossed, I had the feeling that there was something inside the bottle. So I asked her diverse questions of the small-talk league, just to scrabble around the essence of her being. And I shouldn’t have done this. Not in my condition of a busy scientist earning his degrees. Since then, from the very moment I thought she opened her lid, all theories, hypothesis and axioms are gone from my head. So much place might just be about enough to hold the clear beauty inside acetonitrile.
I was determined to break all social barriers that were impeding me to fall in love with her. But disadvantageously, during this short period, which passed as we were still on free-fall, something peculiar and notoriously odd about the behaviour of the amino acids drew our attention from ourselves and focused it on them. Our minds were excited by the unexpected facts revealed to us. But this explanation about the real complex nature of the amino acids would blow the frame of this report... for the moment.
