User:Torsten Waldminghaus/qPCR-Primers: Difference between revisions

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(New page: {| border="3" ! Name !! Sequence !! Characteristics !! |- ! uvrDfw | AGTTCCCGCAGGTGTTTATC | qPCR for uvrD-region with no GATC-sites (Region B from <cite>Yamazoe-2005</cite>) |- ! uvrDrv ...)
 
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! uvrDfw
! uvrDfw
| AGTTCCCGCAGGTGTTTATC
| AGTTCCCGCAGGTGTTTATC
| qPCR for uvrD-region with no GATC-sites (Region B from <cite>Yamazoe-2005</cite>)
| qPCR for uvrD-region on E. coli chromosomewith no GATC-sites (Region B from <cite>Yamazoe-2005</cite>)
|-
|-
! uvrDrv   
! uvrDrv   
|GTCAGCGTCAGTTTCTGCAT
|GTCAGCGTCAGTTTCTGCAT
|qPCR for uvrD-region with no GATC-sites (Region B from <cite>Yamazoe-2005</cite>)
|qPCR for uvrD-region on E. coli chromosomewith no GATC-sites (Region B from <cite>Yamazoe-2005</cite>)
|-
|-
!uvrDprobe   
!uvrDprobe   
|AGACGCCCGCCTTCATCCAG
|AGACGCCCGCCTTCATCCAG
|HPLC-pure 5'Fam - 3'Tamra qPCR for uvrD-region with no GATC-sites (Region B from Yamazoe et al., 200
|HPLC-pure 5'Fam - 3'Tamra qPCR for uvrD-region on E. coli chromosome with no GATC-sites (Region B from <cite>Yamazoe-2005</cite>
|-
! yahEFfw
| CCATCGAGACGATCAAAGAA
| qPCR for yahEF-region on E. coli chromosome with many GATC-sites (Region 2 from <cite>Yamazoe-2005</cite>)
|-
! yahEFrv
| CAGCATCTGGCTTTGTTGTT
| qPCR for yahEF-region on E. coli chromosome with many GATC-sites (Region 2 from <cite>Yamazoe-2005</cite>)
|-
! yahEFprobe
| AACTCGCGTCCTTCGGCAGC
| HPLC5'Fam - 3'Tamra qPCR for yahEF-region on E. coli chromosome with many GATC-sites (Region 2 from <cite>Yamazoe-2005</cite>)
|-
!cluster-fw
|CTGACTGATGAGATCCAACGA
|qPCR for GATC-cluster
!cluster-rv
|CTGGTGCTACGCCTGAATAA
|qPCR for GATC-cluster
!cluster-p
|AAATTCGACCCGGCTGTCGC
|qPCR for GATC-cluster
|}
|}


Revision as of 02:30, 16 December 2008

Name Sequence Characteristics
uvrDfw AGTTCCCGCAGGTGTTTATC qPCR for uvrD-region on E. coli chromosomewith no GATC-sites (Region B from [1])
uvrDrv GTCAGCGTCAGTTTCTGCAT qPCR for uvrD-region on E. coli chromosomewith no GATC-sites (Region B from [1])
uvrDprobe AGACGCCCGCCTTCATCCAG HPLC-pure 5'Fam - 3'Tamra qPCR for uvrD-region on E. coli chromosome with no GATC-sites (Region B from [1]
yahEFfw CCATCGAGACGATCAAAGAA qPCR for yahEF-region on E. coli chromosome with many GATC-sites (Region 2 from [1])
yahEFrv CAGCATCTGGCTTTGTTGTT qPCR for yahEF-region on E. coli chromosome with many GATC-sites (Region 2 from [1])
yahEFprobe AACTCGCGTCCTTCGGCAGC HPLC5'Fam - 3'Tamra qPCR for yahEF-region on E. coli chromosome with many GATC-sites (Region 2 from [1])
cluster-fw CTGACTGATGAGATCCAACGA qPCR for GATC-cluster cluster-rv CTGGTGCTACGCCTGAATAA qPCR for GATC-cluster cluster-p AAATTCGACCCGGCTGTCGC qPCR for GATC-cluster


  1. Yamazoe M, Adachi S, Kanaya S, Ohsumi K, and Hiraga S. Sequential binding of SeqA protein to nascent DNA segments at replication forks in synchronized cultures of Escherichia coli. Mol Microbiol. 2005 Jan;55(1):289-98. DOI:10.1111/j.1365-2958.2004.04389.x | PubMed ID:15612935 | HubMed [Yamazoe-2005]
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