User:Torsten Waldminghaus/protein gel western: Difference between revisions
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(New page: ==Recipe for buffers== {|border="1" |+ '''Resuspension buffer''' |- |20 ml TE buffer |- |5 ml Stacking gel buffer |- |3.5 ml 10% SDS |- |1.5 ml glycerol |- |4 ml ddH<sub>2</sub>O |...) |
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Revision as of 08:05, 8 November 2010
Recipe for buffers
| 20 ml TE buffer |
| 5 ml Stacking gel buffer |
| 3.5 ml 10% SDS |
| 1.5 ml glycerol |
| 4 ml ddH2O |
- Store at rt.
| 285 μL 2.5M DTT |
| 205 μL 10%SDS |
| 10 μL 10% Bromphenolblue |
- Store at -20°C
- Should be 15% of endvolume (for example 42.5 μL sample + 7.5 μL Loading buffer
