User:Torsten Waldminghaus/Strains

Strain	Parental strain	Characteristics	AB resistence	Reference
TWx1	DH5α	supE44 ΔlacU169(Φ80lacZΔM15) hsdR17 recA1 gyrA96	no
TWx2	DH5α	pFH2102	amp
TWx3	MG1655	?	no
TWx4	CM735	metE46 trp-3,his-4,thi-1,galK2,lacY1 or lacZ4,mth-1, ara-9,tsx-3,ton-1,rpsL8 or p,supE44 -	no	[1]
TWx5	DH5α	pGEM-pyrB	amp
TWx6	DH5α	pFH2102-pyrB (=ppyrB)	amp
TWx7	SG2221T	pFH2102	amp	[2]
TWx8	MG1655	pyrB::Tn5	kan	Transfered to MG1655 by P1 transduction from ME8359 (National BioResource Project E.coli Strain)
TWx9	TW8	pyrB::Tn5 ppyrB	kan, amp
TWx10	TW8	pyrB::Tn5 pFH2102 (empty vector)	kan, amp
TWx11	TW3 (MG1655)	ppyrB	amp
TWx12	TW3 (MG1655)	pFH2102 (empty vector)	amp
TWx13	CM735	EBO193 (ΔseqA) (box T24)seqA inframe deletion by pBIP	no
TWx14	DH5α	pET3a-Pfu	amp	strain to overproduce and purify Pfu DNA polymerase
TWx15	DH5α	pTaq	amp	strain to overproduce and purify Taq DNA polymerase
TWx16	KS0003	MG1655 dam16 pGATC	km, cm
TWx17	MG1655	pGATC	cm
TWx18	ALO1826 (box R41)		amp
TWx19	ALO1829 (box R43)		amp, cm
TWx20	MG1655	KS0003 (dam16) (box O33)	km
TWx21	DY330	proAB::GATCcluster	cm
TWx22	DY330	aroA::GATCcluster	cm
TWx23	DY330	ter::GATCcluster	cm
TWx24	DY330	hisA::GATCcluster	cm
TWx25	DY330	srlA::GATCcluster	cm
TWx26	MG1655	proAB::GATCcluster	cm	Transfered to MG1655 by P1 transduction from TWx21; checked for not being DY330 by PCR with primers for λ-beta
TWx27	MG1655	aroA::GATCcluster	cm	Transfered to MG1655 by P1 transduction from TWx22; checked for not being DY330 by PCR with primers for λ-beta
TWx28	MG1655	ter::GATCcluster (1)	cm	Transfered to MG1655 by P1 transduction from TWx23; checked for not being DY330 by PCR with primers for λ-beta; checked by PCR with primers up- and downstream of the chrom. insertion
TWx29	MG1655	ter::GATCcluster (2)	cm	Transfered to MG1655 by P1 transduction from TWx23; checked for not being DY330 by PCR with primers for λ-beta; checked by PCR with primers up- and downstream of the chrom. insertion
TWx30	MG1655	srlA::GATCcluster	cm	Transfered to MG1655 by P1 transduction from TWx25; checked for not being DY330 by PCR with primers for λ-beta
TWx31	MG1655	hisA:GATCcluster	cm	Transfered to MG1655 by P1 transduction from TWx24; checked for not being DY330 by PCR with primers for λ-beta
TWx32	CM735	NK9386 Ptac::fliCst	no	strain with IPTG inducible stick flagella for baby cell column [3]
TWx33	MG1655	ter::GATCcluster (ter1)	cm	Transfered to MG1655 by P1 transduction from TWx23 but only grown on AB-acetate medium in case fast growth are a problem to this strain (TWx28 and TWx29 are in principle the same but have been grown in LB; checked for not being DY330 by PCR with primers for λ-beta; checked by PCR with primers up- and downstream of the chrom. insertion
TWx34	MG1655	ter::GATCcluster (ter2)	cm	Transfered to MG1655 by P1 transduction from TWx23 but only grown on AB-acetate medium in case fast growth are a problem to this strain (TWx28 and TWx29 are in principle the same but have been grown in LB; checked for not being DY330 by PCR with primers for λ-beta; checked by PCR with primers up- and downstream of the chrom. insertion
TWx35	MG1655	ALO1739 (arok17::cat/pMS2)	cm, km	strain with less Dam molecules per cell and longer hemimethylation due to polar effects on dam expression. Used in [4]
TWx36		GM3819 (F- dam-16::Kan thr-1 leuB6 thi-1 argE3 hisG4 proA2 lacY1 galK2 mtl-1 xyl-5 ara-14 rpsL31 tsx-33 supE44 rfbD1 kdgK51)	km	The prototype dam-16::Kan deletion strain [5] http://users.umassmed.edu/martin.marinus/dstrains.html
TWx37	GM3819	pMQ430/GM3819	km, amp	pMQ430 is a pBAD18 derivative with the cloned dam gene, without its ribosome binding site, under control of the pBAD promoter. Expression of dam occurs in the presence of arabinose but not in its absence [6] http://users.umassmed.edu/martin.marinus/plasmid.html
TWx38	Agrobacterium tumefaciens C58			wt
TWx39	Agrobacterium tumefaciens A136	rif		Ti plasmid-cured derivative of strain C58
TWx40	Agrobacterium tumefaciens UBAPF I	rif		Plasmid free strain [7]
TWx41	Agrobacterium tumefaciens UBAPF II	rif		Plasmid free strain [7]
TWx42	Agrobacterium tumefaciens UBAPF I	pTrc200	rif, strep, spec
TWx43	Agrobacterium tumefaciens UBAPF I	pTrc200_dnaA	rif, strep, spec	IPTG inducible dnaA
TWx44	Agrobacterium tumefaciens UBAPF I	pTrc200_dnaA_his	rif, strep, spec	IPTG inducible dnaA with his tag
TWx45	Agrobacterium tumefaciens UBAPF II	pTrc200	rif, strep, spec
TWx46	Agrobacterium tumefaciens UBAPF II	pTrc200_dnaA	rif, strep, spec	IPTG inducible dnaA
TWx47	Agrobacterium tumefaciens UBAPF II	pTrc200_dnaA_his	rif, strep, spec	IPTG inducible dnaA with his tag
TWx48	MG1655 dnaC2 (R34)	dnaC2 pMQ430	amp, tet	For pMQ430 see TWx37
TWx49	MG1655	KS9921 (O19) delta seqA
TWx50	TWX49 (KS9921)	ter::GATC-cluster	Transfered by P1 transduction from TWx23
TWx51	TWX50	ter::GATC-cluster; pIO4	seqA4 plasmid, IPTG inducible

2. Hoskins JR, Singh SK, Maurizi MR, and Wickner S. Protein binding and unfolding by the chaperone ClpA and degradation by the protease ClpAP. Proc Natl Acad Sci U S A. 2000 Aug 1;97(16):8892-7. DOI:10.1073/pnas.97.16.8892 |
3. Bates D, Epstein J, Boye E, Fahrner K, Berg H, and Kleckner N. The Escherichia coli baby cell column: a novel cell synchronization method provides new insight into the bacterial cell cycle. Mol Microbiol. 2005 Jul;57(2):380-91. DOI:10.1111/j.1365-2958.2005.04693.x |
4. Løbner-Olesen A, Marinus MG, and Hansen FG. Role of SeqA and Dam in Escherichia coli gene expression: a global/microarray analysis. Proc Natl Acad Sci U S A. 2003 Apr 15;100(8):4672-7. DOI:10.1073/pnas.0538053100 |
5. Parker B and Marinus MG. A simple and rapid method to obtain substitution mutations in Escherichia coli: isolation of a dam deletion/insertion mutation. Gene. 1988 Dec 20;73(2):531-5. DOI:10.1016/0378-1119(88)90517-3 |
6. Calmann MA and Marinus MG. Regulated expression of the Escherichia coli dam gene. J Bacteriol. 2003 Aug;185(16):5012-4. DOI:10.1128/JB.185.16.5012-5014.2003 |
7. Hynes MF, Simon R, and Pühler A. The development of plasmid-free strains of Agrobacterium tumefaciens by using incompatibility with a Rhizobium meliloti plasmid to eliminate pAtC58. Plasmid. 1985 Mar;13(2):99-105. DOI:10.1016/0147-619x(85)90062-9 |
8. Hansen EB, Atlung T, Hansen FG, Skovgaard O, and von Meyenburg K. Fine structure genetic map and complementation analysis of mutations in the dnaA gene of Escherichia coli. Mol Gen Genet. 1984;196(3):387-96. DOI:10.1007/BF00436184 |