User:Torsten Waldminghaus/Strains: Difference between revisions
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! Strain !! Parental strain !! Characteristics !! AB resistence !! Reference | ! Strain !! Parental strain !! Characteristics !! AB resistence !! Reference | ||
|- | |- | ||
! | ! TWx1 | ||
| DH5α | | DH5α | ||
| supE44 ΔlacU169(Φ80lacZΔM15) hsdR17 recA1 gyrA96 | | supE44 ΔlacU169(Φ80lacZΔM15) hsdR17 recA1 gyrA96 | ||
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| | | | ||
|- | |- | ||
! | ! TWx2 | ||
|DH5α | |DH5α | ||
|pFH2102 | |pFH2102 | ||
| Line 14: | Line 14: | ||
| | | | ||
|- | |- | ||
! | ! TWx3 | ||
|MG1655 | |MG1655 | ||
|? | |? | ||
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| | | | ||
|- | |- | ||
! | ! TWx4 | ||
|CM735 | |CM735 | ||
|metE46 trp-3,his-4,thi-1,galK2,lacY1 or lacZ4,mth-1, ara-9,tsx-3,ton-1,rpsL8 or p,supE44 - | |metE46 trp-3,his-4,thi-1,galK2,lacY1 or lacZ4,mth-1, ara-9,tsx-3,ton-1,rpsL8 or p,supE44 - | ||
| Line 26: | Line 26: | ||
|<cite>Hansen-1989</cite> | |<cite>Hansen-1989</cite> | ||
|- | |- | ||
! | ! TWx5 | ||
|DH5α | |DH5α | ||
|pGEM-pyrB | |pGEM-pyrB | ||
| Line 32: | Line 32: | ||
| | | | ||
|- | |- | ||
! | ! TWx6 | ||
|DH5α | |DH5α | ||
|pFH2102-pyrB (=ppyrB) | |pFH2102-pyrB (=ppyrB) | ||
| Line 38: | Line 38: | ||
| | | | ||
|- | |- | ||
! | ! TWx7 | ||
|SG2221T | |SG2221T | ||
|pFH2102 | |pFH2102 | ||
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|<cite>Hoskins-2000</cite> | |<cite>Hoskins-2000</cite> | ||
|- | |- | ||
! | ! TWx8 | ||
|MG1655 | |MG1655 | ||
|pyrB::Tn5 | |pyrB::Tn5 | ||
| Line 50: | Line 50: | ||
|Transfered to MG1655 by P1 transduction from ME8359 ([http://www.shigen.nig.ac.jp/ecoli/strain/top/top.jsp National BioResource Project E.coli Strain]) | |Transfered to MG1655 by P1 transduction from ME8359 ([http://www.shigen.nig.ac.jp/ecoli/strain/top/top.jsp National BioResource Project E.coli Strain]) | ||
|- | |- | ||
! | ! TWx9 | ||
|TW8 | |TW8 | ||
|pyrB::Tn5 ppyrB | |pyrB::Tn5 ppyrB | ||
| Line 56: | Line 56: | ||
| | | | ||
|- | |- | ||
! | ! TWx10 | ||
|TW8 | |TW8 | ||
|pyrB::Tn5 pFH2102 (empty vector) | |pyrB::Tn5 pFH2102 (empty vector) | ||
| Line 62: | Line 62: | ||
| | | | ||
|- | |- | ||
! | ! TWx11 | ||
|TW3 (MG1655) | |TW3 (MG1655) | ||
|ppyrB | |ppyrB | ||
| Line 68: | Line 68: | ||
| | | | ||
|- | |- | ||
! | ! TWx12 | ||
|TW3 (MG1655) | |TW3 (MG1655) | ||
|pFH2102 (empty vector) | |pFH2102 (empty vector) | ||
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| | | | ||
|- | |- | ||
! | ! TWx13 | ||
| | | | ||
| | | | ||
| Line 80: | Line 80: | ||
| | | | ||
|- | |- | ||
! | ! TWx14 | ||
| | | | ||
| | | | ||
| Line 86: | Line 86: | ||
| | | | ||
|- | |- | ||
! | ! TWx15 | ||
| | | | ||
| | | | ||
| Line 92: | Line 92: | ||
| | | | ||
|- | |- | ||
! | ! TWx16 | ||
|KS0006 | |KS0006 | ||
|MG1655 Δdam | |MG1655 Δdam | ||
| Line 98: | Line 98: | ||
|no | |no | ||
|- | |- | ||
! | ! TWx17 | ||
|MG1655 | |MG1655 | ||
|pGATC | |pGATC | ||
| Line 104: | Line 104: | ||
| | | | ||
|- | |- | ||
! | ! TWx18 | ||
|ALO1826 (box R41) | |ALO1826 (box R41) | ||
| | | | ||
| Line 110: | Line 110: | ||
| | | | ||
|- | |- | ||
! | ! TWx19 | ||
|ALO1829 (box R43) | |ALO1829 (box R43) | ||
| | | | ||
Revision as of 01:20, 11 December 2008
| Strain | Parental strain | Characteristics | AB resistence | Reference |
|---|---|---|---|---|
| TWx1 | DH5α | supE44 ΔlacU169(Φ80lacZΔM15) hsdR17 recA1 gyrA96 | no | |
| TWx2 | DH5α | pFH2102 | amp | |
| TWx3 | MG1655 | ? | no | |
| TWx4 | CM735 | metE46 trp-3,his-4,thi-1,galK2,lacY1 or lacZ4,mth-1, ara-9,tsx-3,ton-1,rpsL8 or p,supE44 - | no | [1] |
| TWx5 | DH5α | pGEM-pyrB | amp | |
| TWx6 | DH5α | pFH2102-pyrB (=ppyrB) | amp | |
| TWx7 | SG2221T | pFH2102 | amp | [2] |
| TWx8 | MG1655 | pyrB::Tn5 | kan | Transfered to MG1655 by P1 transduction from ME8359 (National BioResource Project E.coli Strain) |
| TWx9 | TW8 | pyrB::Tn5 ppyrB | kan, amp | |
| TWx10 | TW8 | pyrB::Tn5 pFH2102 (empty vector) | kan, amp | |
| TWx11 | TW3 (MG1655) | ppyrB | kan, amp | |
| TWx12 | TW3 (MG1655) | pFH2102 (empty vector) | kan, amp | |
| TWx13 | ||||
| TWx14 | ||||
| TWx15 | ||||
| TWx16 | KS0006 | MG1655 Δdam | no | no |
| TWx17 | MG1655 | pGATC | cm | |
| TWx18 | ALO1826 (box R41) | amp | ||
| TWx19 | ALO1829 (box R43) | amp, cm |
- Hoskins JR, Singh SK, Maurizi MR, and Wickner S. Protein binding and unfolding by the chaperone ClpA and degradation by the protease ClpAP. Proc Natl Acad Sci U S A. 2000 Aug 1;97(16):8892-7. DOI:10.1073/pnas.97.16.8892 |
- Hansen EB, Atlung T, Hansen FG, Skovgaard O, and von Meyenburg K. Fine structure genetic map and complementation analysis of mutations in the dnaA gene of Escherichia coli. Mol Gen Genet. 1984;196(3):387-96. DOI:10.1007/BF00436184 |
