User:Torsten Waldminghaus/Primer
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- All primers are in concentrations of 100pmol/μL
| Name | Sequence | Characteristics | Primer number |
|---|---|---|---|
| GFPmut7fw | GTTATCCGGACCATATGAAACGGC | 5’-phosph. HPLC | 1 |
| GFPmut8fw | CATTGAAGATGGGTCCGTTCAACTAG | 5’-phosph. HPLC | 2 |
| GFPmut9fw | CCTTTCGAAAGACCCCAACGAAAAG | 5’-phosph. HPLC | 3 |
| MseIlong | AGTGGGATTCCGCATGCTAGT | 4 | |
| MseIshort | TAACTAGCATCG | This sequence seems to be wrong because the last CG is not complementary to MseIlong but should instead be GC => use MseIshortnew instead | 5 |
| MseIshortnew | TAACTAGCATGC | 6 | |
| MseIshortnewNo | TAACTAGCATGC | Not phosphorilated | 7 |
| GATC19DNAfw | GCCCGCGGATCCGCCCGCC | oligo for methylation experiment from A. Humeny et al. 2003 | |
| GATC19DNArv | GGCGGGCGGATCCGCGGGC | oligo for methylation experiment from A. Humeny et al. 2003 | |
| GATC19RNAfw | GCCCGCGGAUCCGCCCGCC | RNA oligo for methylation experiment from A. Humeny et al. 2003 | |
| GATC19RNArv | GGCGGGCGGAUCCGCGGGC | RNA oligo for methylation experiment from A. Humeny et al. 2003 | |
| bet-fw | GTCGACCCACAGGAACTGAT | To distinguish DY330 (with lambda phage) from other E. coli strains use primers for bet as part of the recombination machinery of lambda | |
| bet-rev | GGCTGACGTTCTGCAGTGTA | To distinguish DY330 (with lambda phage) from other E. coli strains use primers for bet as part of the recombination machinery of lambda | |
| Cm_seqF | |||
| Cm_seqR | |||
| Cut-test_fw | TAATAGGCATGCTAGCAGCTGAGCGTAGCTAGCGATGCAACGACGGTGATCAGCGTCGATGCAGCCGAAACGATGACTGTCACATAGCTGATGCTTTGCT | ||
| Cut_test_rv | TAAGCAAAGCATCAGCTATGTGACAGTCATCGTTTCGGCTGCATCGACGAAGATCACCGTCGTTGCATCGCTAGCTACGCTCAGCTGCTAGCATGCCTAT | ||
