User:Torsten Waldminghaus/Primer: Difference between revisions
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! MseIshortnewNo | |||
|TAACTAGCATGC | |||
|Not phosphorilated | |||
|7 | |||
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! GATC19DNAfw | ! GATC19DNAfw | ||
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| GGCTGACGTTCTGCAGTGTA | | GGCTGACGTTCTGCAGTGTA | ||
|To distinguish DY330 (with lambda phage) from other E. coli strains use primers for bet as part of the recombination machinery of lambda | |To distinguish DY330 (with lambda phage) from other E. coli strains use primers for bet as part of the recombination machinery of lambda | ||
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! Cm_seqF | |||
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! Cm_seqR | |||
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! Cut-test_fw | |||
| TAATAGGCATGCTAGCAGCTGAGCGTAGCTAGCGATGCAACGACGGTGATCAGCGTCGATGCAGCCGAAACGATGACTGTCACATAGCTGATGCTTTGCT | |||
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! Cut_test_rv | |||
| TAAGCAAAGCATCAGCTATGTGACAGTCATCGTTTCGGCTGCATCGACGAAGATCACCGTCGTTGCATCGCTAGCTACGCTCAGCTGCTAGCATGCCTAT | |||
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Revision as of 02:58, 12 August 2011
- All primers are in concentrations of 100pmol/μL
| Name | Sequence | Characteristics | Primer number |
|---|---|---|---|
| GFPmut7fw | GTTATCCGGACCATATGAAACGGC | 5’-phosph. HPLC | 1 |
| GFPmut8fw | CATTGAAGATGGGTCCGTTCAACTAG | 5’-phosph. HPLC | 2 |
| GFPmut9fw | CCTTTCGAAAGACCCCAACGAAAAG | 5’-phosph. HPLC | 3 |
| MseIlong | AGTGGGATTCCGCATGCTAGT | 4 | |
| MseIshort | TAACTAGCATCG | This sequence seems to be wrong because the last CG is not complementary to MseIlong but should instead be GC => use MseIshortnew instead | 5 |
| MseIshortnew | TAACTAGCATGC | 6 | |
| MseIshortnewNo | TAACTAGCATGC | Not phosphorilated | 7 |
| GATC19DNAfw | GCCCGCGGATCCGCCCGCC | oligo for methylation experiment from A. Humeny et al. 2003 | |
| GATC19DNArv | GGCGGGCGGATCCGCGGGC | oligo for methylation experiment from A. Humeny et al. 2003 | |
| GATC19RNAfw | GCCCGCGGAUCCGCCCGCC | RNA oligo for methylation experiment from A. Humeny et al. 2003 | |
| GATC19RNArv | GGCGGGCGGAUCCGCGGGC | RNA oligo for methylation experiment from A. Humeny et al. 2003 | |
| bet-fw | GTCGACCCACAGGAACTGAT | To distinguish DY330 (with lambda phage) from other E. coli strains use primers for bet as part of the recombination machinery of lambda | |
| bet-rev | GGCTGACGTTCTGCAGTGTA | To distinguish DY330 (with lambda phage) from other E. coli strains use primers for bet as part of the recombination machinery of lambda | |
| Cm_seqF | |||
| Cm_seqR | |||
| Cut-test_fw | TAATAGGCATGCTAGCAGCTGAGCGTAGCTAGCGATGCAACGACGGTGATCAGCGTCGATGCAGCCGAAACGATGACTGTCACATAGCTGATGCTTTGCT | ||
| Cut_test_rv | TAAGCAAAGCATCAGCTATGTGACAGTCATCGTTTCGGCTGCATCGACGAAGATCACCGTCGTTGCATCGCTAGCTACGCTCAGCTGCTAGCATGCCTAT | ||
