User:Tom Adie/Sandbox/1: Difference between revisions
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Revision as of 17:53, 19 July 2009
<html><TABLE WIDTH="80%" ><TR ><TD ><A NAME="TBL1" > </A ></TD ></TR ><TR ><TD ALIGN="CENTER" ><B >Table 1. </B ><B >Strains and plasmids used in this work.</B ></TD ></TR ><TR VALIGN="BOTTOM" ><TD ><HR size="2" noshade="1"></TD ></TR ><TR ><TD ALIGN="CENTER" ><TABLE ><TR ><TH ALIGN="left" VALIGN="TOP" ><P>Strain or plasmid</TH ><TH ALIGN="left" VALIGN="TOP" ><P>Relevant properties or genotype</TH ><TH ALIGN="left" VALIGN="TOP" ><NOBR ><P>Source</NOBR ></TH ></TR ><TR ><TD COLSPAN="3" ><HR size="1"></TD ></TR ><TR ><TD ALIGN="left" VALIGN="TOP" ><NOBR ><P>Strains</NOBR ></TD ><TD ALIGN="left" VALIGN="TOP" ><NOBR ></NOBR ></TD ><TD ALIGN="left" VALIGN="TOP" ><NOBR ></NOBR ></TD ></TR ><TR ><TD ALIGN="left" VALIGN="TOP" ><P><I >E. coli</I > DH5<IMG SRC="/giflibrary/12/alpha.gif" BORDER="0"></TD ><TD ALIGN="left" VALIGN="TOP" ><P>F<SUP >-</SUP >, <I ><IMG SRC="/giflibrary/12/phgr.gif" BORDER="0" ALIGN="ABSBOTTOM"></I >80d, <I >lacZ</I ><IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">M15, <I >endA</I >1, <I >recA</I >1, <I >hsdR</I >17(r<SUB >K</SUB ><SUP >-</SUP >m<SUB >K</SUB ><SUP >-</SUP >), <I >supE</I >44, <I >thi</I >-1, <I >gyrA</I >96, <I >relA</I >1, <IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">(<I >lacZYA-argF</I >)U169</TD ><TD ALIGN="left" VALIGN="TOP" ><NOBR ><P>[a]</NOBR ></TD ></TR ><TR ><TD ALIGN="left" VALIGN="TOP" ><P><I >E. coli</I > M15 [pREP4]</TD ><TD ALIGN="left" VALIGN="TOP" ><P>F<SUP >-</SUP >, <I >lacZ<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">M15, thi</I >-1, <I >lac<SUP >-</SUP ></I >, <I >mtl<SUP >-</SUP ></I >, <I >recA<SUP >+</SUP ></I > , Km<SUP >R</SUP ></TD ><TD ALIGN="left" VALIGN="TOP" ><NOBR ><P>[a]</NOBR ></TD ></TR ><TR ><TD ALIGN="left" VALIGN="TOP" ><P><I >Pseudomonas putida</I > KT2440</TD ><TD ALIGN="left" VALIGN="TOP" ><P>type strain</TD ><TD ALIGN="left" VALIGN="TOP" ><NOBR ><P>[b]</NOBR ></TD ></TR ><TR ><TD ALIGN="left" VALIGN="TOP" ><P><I >Synechocystis</I > sp. PCC 6803</TD ><TD ALIGN="left" VALIGN="TOP" ><P>type strain</TD ><TD ALIGN="left" VALIGN="TOP" ><NOBR ><P>[c]</NOBR ></TD ></TR ><TR ><TD ALIGN="left" VALIGN="TOP" ><NOBR ><P>Plasmids</NOBR ></TD ><TD ALIGN="left" VALIGN="TOP" ><NOBR ></NOBR ></TD ><TD ALIGN="left" VALIGN="TOP" ><NOBR ></NOBR ></TD ></TR ><TR ><TD ALIGN="left" VALIGN="TOP" ><NOBR ><P>pCAR16</NOBR ></TD ><TD ALIGN="left" VALIGN="TOP" ><P><IMG SRC="/giflibrary/12/beta.gif" BORDER="0" ALIGN="ABSBOTTOM">-carotene biosynthesis gene cluster from <I >Pantoea ananatis</I > in pUC19, Amp<SUP >R</SUP ></TD ><TD ALIGN="left" VALIGN="TOP" ><NOBR ><P>[<A HREF="#BIB27" >27</A >]</NOBR ></TD ></TR ><TR ><TD ALIGN="left" VALIGN="TOP" ><NOBR ><P>pJF119EH</NOBR ></TD ><TD ALIGN="left" VALIGN="TOP" ><P>cloning vector, tac-promoter, IPTG inducible, Amp<SUP >R</SUP ></TD ><TD ALIGN="left" VALIGN="TOP" ><NOBR ><P>[<A HREF="#BIB25" >25</A >]</NOBR ></TD ></TR ><TR ><TD ALIGN="left" VALIGN="TOP" ><NOBR ><P>pJF119<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">N</NOBR ></TD ><TD ALIGN="left" VALIGN="TOP" ><P>cloning vector, RBS, tac-promoter, IPTG inducible, Amp<SUP >R</SUP ></TD ><TD ALIGN="left" VALIGN="TOP" ><P>this study</TD ></TR ><TR ><TD ALIGN="left" VALIGN="TOP" ><NOBR ><P>pAW229</NOBR ></TD ><TD ALIGN="left" VALIGN="TOP" ><P>cloning vector, RBS, <I >rha</I >-promoter, <FONT SIZE="-1" >L</FONT >-rhamnose inducible, Cm<SUP >R</SUP ></TD ><TD ALIGN="left" VALIGN="TOP" ><NOBR ><P>[<A HREF="#BIB26" >26</A >]</NOBR ></TD ></TR ><TR ><TD ALIGN="left" VALIGN="TOP" ><NOBR ><P>pQE31-VTE1</NOBR ></TD ><TD ALIGN="left" VALIGN="TOP" ><P>tocopherol-cyclase (vte1) from <I >Arabidopsis thaliana</I > in pQE31, IPTG inducible, Amp<SUP >R</SUP ></TD ><TD ALIGN="left" VALIGN="TOP" ><NOBR ><P>[<A HREF="#BIB18A" >18a</A >]</NOBR ></TD ></TR ><TR ><TD ALIGN="left" VALIGN="TOP" ><NOBR ><P>pCAS2JF</NOBR ></TD ><TD ALIGN="left" VALIGN="TOP" ><P>1.2 kb EcoRI/BamHI PCR fragment <I >hpd</I > in pJF119EH</TD ><TD ALIGN="left" VALIGN="TOP" ><P>this study</TD ></TR ><TR ><TD ALIGN="left" VALIGN="TOP" ><NOBR ><P>pCAS8</NOBR ></TD ><TD ALIGN="left" VALIGN="TOP" ><P>1.1 kb NdeI/BamHI PCR fragment <I >ggh</I > in pJF119<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">N</TD ><TD ALIGN="left" VALIGN="TOP" ><P>this study</TD ></TR ><TR ><TD ALIGN="left" VALIGN="TOP" ><NOBR ><P>pCAS11</NOBR ></TD ><TD ALIGN="left" VALIGN="TOP" ><P>0.9 kb BglII/BamHI PCR fragment <I >crtE</I > in pCAS8</TD ><TD ALIGN="left" VALIGN="TOP" ><P>this study</TD ></TR ><TR ><TD ALIGN="left" VALIGN="TOP" ><NOBR ><P>pCAS12</NOBR ></TD ><TD ALIGN="left" VALIGN="TOP" ><P>1.0 kb BglII/BamHI PCR fragment <I >hpt</I > in pCAS11</TD ><TD ALIGN="left" VALIGN="TOP" ><P>this study</TD ></TR ><TR ><TD ALIGN="left" VALIGN="TOP" ><NOBR ><P>pCAS15</NOBR ></TD ><TD ALIGN="left" VALIGN="TOP" ><P>1.2 kb NdeI/BamHI PCR fragment <I >hpd</I > in pAW229</TD ><TD ALIGN="left" VALIGN="TOP" ><P>this study</TD ></TR ><TR ><TD ALIGN="left" VALIGN="TOP" ><NOBR ><P>pCAS18</NOBR ></TD ><TD ALIGN="left" VALIGN="TOP" ><P>1.2 kb BglII/BamHI PCR fragment <I >hpd</I > in pCAS11</TD ><TD ALIGN="left" VALIGN="TOP" ><P>this study</TD ></TR ><TR ><TD ALIGN="left" VALIGN="TOP" ><NOBR ><P>pCAS19</NOBR ></TD ><TD ALIGN="left" VALIGN="TOP" ><P>1.0 kb NdeI/BamHI PCR fragment <I >hpt</I > in pAW229</TD ><TD ALIGN="left" VALIGN="TOP" ><P>this study</TD ></TR ><TR ><TD ALIGN="left" VALIGN="TOP" ><NOBR ><P>pCAS23</NOBR ></TD ><TD ALIGN="left" VALIGN="TOP" ><P>1.0 kb NdeI/BamHI PCR fragment <I >hpt</I > in pJF119<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">N</TD ><TD ALIGN="left" VALIGN="TOP" ><P>this study</TD ></TR ><TR ><TD ALIGN="left" VALIGN="TOP" ><NOBR ><P>pCAS24</NOBR ></TD ><TD ALIGN="left" VALIGN="TOP" ><P>0.9 kb NdeI/BamHI PCR fragment <I >crtE</I > in pCAS23</TD ><TD ALIGN="left" VALIGN="TOP" ><P>this study</TD ></TR ><TR ><TD ALIGN="left" VALIGN="TOP" ><NOBR ><P>pCAS27</NOBR ></TD ><TD ALIGN="left" VALIGN="TOP" ><P>1.1 kb NdeI/BamHI PCR fragment <I >cyc-sy</I > in pAW229</TD ><TD ALIGN="left" VALIGN="TOP" ><P>this study</TD ></TR ><TR ><TD ALIGN="left" VALIGN="TOP" ><NOBR ><P>pCAS29</NOBR ></TD ><TD ALIGN="left" VALIGN="TOP" ><P>1.2 kb BglII/BamHI PCR fragment <I >hpd</I > in pCAS18</TD ><TD ALIGN="left" VALIGN="TOP" ><P>this study</TD ></TR ><TR ><TD ALIGN="left" VALIGN="TOP" ><NOBR ><P>pCAS30</NOBR ></TD ><TD ALIGN="left" VALIGN="TOP" ><P>0.9 kb NdeI/BamHI PCR fragment <I >crtE</I > in pJF119<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">N</TD ><TD ALIGN="left" VALIGN="TOP" ><P>this study</TD ></TR ><TR ><TD ALIGN="left" VALIGN="TOP" ><NOBR ><P>pCAS37</NOBR ></TD ><TD ALIGN="left" VALIGN="TOP" ><P>1.2 kb BglII/BamHI PCR fragment <I >hpd</I > in pCAS24</TD ><TD ALIGN="left" VALIGN="TOP" ><P>this study</TD ></TR ><TR ><TD ALIGN="left" VALIGN="TOP" ><NOBR ><P>pCAS47</NOBR ></TD ><TD ALIGN="left" VALIGN="TOP" ><P>1.3 kb BglII PCR fragment <I >vte1</I > in pCAS29</TD ><TD ALIGN="left" VALIGN="TOP" ><P>this study</TD ></TR ></TABLE ></TD ></TR ><TR VALIGN="BOTTOM" ><TD ><HR size="2" noshade="1"></TD ></TR ></TABLE ></CENTER ><CENTER ><TABLE WIDTH="80%" ><TR ><TD ><FONT SIZE="-1" > [a] Commercial strain. [b] Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ). [c] A kind gift from Prof. Forchhammer, Universität-Gießen (Germany).<BR></FONT ></TD ></TR ></TABLE ></html>
