User:Tobias Wolfgang Giessen

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Contact Info

Tobias Wolfgang Giessen

• Tobias Wolfgang Giessen
• Research Fellow
• Harvard Medical School
• Department of Systems Biology
• 200 Longwood Avenue
• Boston, MA
• Email me through OpenWetWare

I work in the lab of Pam Silver at Harvard Medical School as a Research Fellow.

Education

• 2013, PhD, Department of Chemistry, Philipps-University Marburg, Marahiel Group
• 2010, MS, Department of Chemistry, Philipps-University Marburg, Marahiel Group
• 2008, Research Internship, Imperial College London, Armstrong Group

Interests

1. Natural Product Biosynthesis
2. Enzymology
3. Synthetic Biology
4. Metabolic Engineering
5. Protein Engineering
6. Carboxysome Assembly
7. Combinatorial Biosynthesis

Publications

1. Giessen TW, Kraas FI, and Marahiel MA. A four-enzyme pathway for 3,5-dihydroxy-4-methylanthranilic acid formation and incorporation into the antitumor antibiotic sibiromycin. Biochemistry. 2011 Jun 28;50(25):5680-92. DOI:10.1021/bi2006114 |
2. Kraas FI, Giessen TW, and Marahiel MA. Exploring the mechanism of lipid transfer during biosynthesis of the acidic lipopeptide antibiotic CDA. FEBS Lett. 2012 Feb 3;586(3):283-8. DOI:10.1016/j.febslet.2012.01.003 |
3. Giessen TW and Marahiel MA. Ribosome-independent biosynthesis of biologically active peptides: Application of synthetic biology to generate structural diversity. FEBS Lett. 2012 Jul 16;586(15):2065-75. DOI:10.1016/j.febslet.2012.01.017 |
4. Bosello M, Mielcarek A, Giessen TW, and Marahiel MA. An enzymatic pathway for the biosynthesis of the formylhydroxyornithine required for rhodochelin iron coordination. Biochemistry. 2012 Apr 10;51(14):3059-66. DOI:10.1021/bi201837f |
5. Giessen TW, Franke KB, Knappe TA, Kraas FI, Bosello M, Xie X, Linne U, and Marahiel MA. Isolation, structure elucidation, and biosynthesis of an unusual hydroxamic acid ester-containing siderophore from Actinosynnema mirum. J Nat Prod. 2012 May 25;75(5):905-14. DOI:10.1021/np300046k |
6. Flühe L, Burghaus O, Wieckowski BM, Giessen TW, Linne U, and Marahiel MA. Two [4Fe-4S] clusters containing radical SAM enzyme SkfB catalyze thioether bond formation during the maturation of the sporulation killing factor. J Am Chem Soc. 2013 Jan 23;135(3):959-62. DOI:10.1021/ja310542g |
7. Giessen TW, von Tesmar AM, and Marahiel MA. A tRNA-dependent two-enzyme pathway for the generation of singly and doubly methylated ditryptophan 2,5-diketopiperazines. Biochemistry. 2013 Jun 18;52(24):4274-83. DOI:10.1021/bi4004827 |
8. Giessen TW, von Tesmar AM, and Marahiel MA. Insights into the generation of structural diversity in a tRNA-dependent pathway for highly modified bioactive cyclic dipeptides. Chem Biol. 2013 Jun 20;20(6):828-38. DOI:10.1016/j.chembiol.2013.04.017 |
9. Giessen TW and Marahiel MA. The tRNA-dependent biosynthesis of modified cyclic dipeptides. Int J Mol Sci. 2014 Aug 21;15(8):14610-31. DOI:10.3390/ijms150814610 |

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