User:Tk/Notebook/MF/2010/06/12

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Mesoplasma florum simplification <html><img src="/images/9/94/Report.png" border="0" /></html> Main Page
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Desired parts

  • Mflorum optimized genes
    • CFP
    • YFP
    • RFP
    • LacZ
    • cat (probably from Sebastian)
    • I-sceI enzyme
  • Cre-lox sites (maybe from Sebastian)
  • I-sceI cut site
  • E. coli optimized mfl304
  • modified pheS (already exists?)
  • up and down sequences around RE gene sites
  • modified plasmid containing left and right transposon sites (M1000 and M1001) with pvuII sites

Eliminate sites for PvuII, NheI, bibrick sites,


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