User:Tk/Notebook/MF/2009/03/23: Difference between revisions
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(Autocreate 2009/03/23 Entry for User:Tk/Notebook/MF) |
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== | ==Genomic DNA Prep== | ||
* | * all cultures grew | ||
* plate out cultures on 1161 plates w/o antibiotic | |||
* prep genomic DNA from 12 ml cultures | |||
* save 20% glycerol stocks of all cultures | |||
* potential problem: overgrowth of culture may have killed some of the cultures | |||
* Use OWW CTAB protocol for genomic DNA scaled up 6x | |||
* 3.6 ml TE | |||
* 18 ul of proteinase K | |||
* 90 ul of 20% SDS (instead of 10%) | |||
* problem resuspending the pellets | |||
* | |||
Latest revision as of 10:35, 24 March 2009
 Mesoplasma florum simplification
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Genomic DNA Prep
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