User:Tk/Notebook/MF/2009/03/21: Difference between revisions

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Choose GF1, W20, Me. entomophilum strain as the ones for next gen sequencing.
Choose GF1, W20, Me. entomophilum strain as the ones for next gen sequencing.


Prepare seed cultures of L1, GF1, W20, M. entomophilum
Prepare seed cultures of L1, GF1, PP1, W20, M. entomophilum




Revision as of 15:17, 21 March 2009

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RNAse H genes

Kochiwa07 reports on the variety of RNAse H genes in bacterial species. He finds three major categories, RNAseH-I, RNAseH-II, and RNAse-III. MF has RNAseH-I and RNAse-II, but the -I has additional domain dsRHbd (double stranded RNA helix binding domain). The genes in MF for these two are:

  • -I (with dsRHbd) is Mfl475, and is apparently essential
  • -II is Mfl534, and has been knocked out several times.

Made new 1161 plates - no antibiotic

Choose GF1, W20, Me. entomophilum strain as the ones for next gen sequencing.

Prepare seed cultures of L1, GF1, PP1, W20, M. entomophilum




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