User:Tk/Notebook/MF/2009/03/21: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(Autocreate 2009/03/21 Entry for User:Tk/Notebook/MF) |
|||
| (5 intermediate revisions by the same user not shown) | |||
| Line 6: | Line 6: | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==RNAse H genes; Genomic DNA prep for Illumina sequencing== | ||
* | Kochiwa07 reports on the variety of RNAse H genes in bacterial species. He finds three major categories, RNAseH-I, RNAseH-II, and RNAse-III. MF has RNAseH-I and RNAse-II, but the -I has additional domain dsRHbd (double stranded RNA helix binding domain). The genes in MF for these two are: | ||
* -I (with dsRHbd) is Mfl475, and is apparently essential | |||
* -III is Mfl537, and has been knocked out several times. | |||
Made new 1161 plates - no antibiotic | |||
Choose GF1, W20, Me. entomophilum strain as the ones for next gen sequencing. | |||
Prepare seed cultures of L1, GF1, PP1, W12, M. entomophilum | |||
Latest revision as of 12:10, 8 May 2012
 Mesoplasma florum simplification
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main Page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
RNAse H genes; Genomic DNA prep for Illumina sequencingKochiwa07 reports on the variety of RNAse H genes in bacterial species. He finds three major categories, RNAseH-I, RNAseH-II, and RNAse-III. MF has RNAseH-I and RNAse-II, but the -I has additional domain dsRHbd (double stranded RNA helix binding domain). The genes in MF for these two are:
Made new 1161 plates - no antibiotic Choose GF1, W20, Me. entomophilum strain as the ones for next gen sequencing. Prepare seed cultures of L1, GF1, PP1, W12, M. entomophilum
| |
