User:Tk/Notebook/MF-xfm/2008/04/21: Difference between revisions
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== | ==Transformation results== | ||
* | * E. coli transformation was normal 200+ colonies | ||
* picked six more colonies on replated osmolarity tests | |||
==recT gene PCR worked, expected size== | |||
* Phusion in 100 ul final | |||
** 50 ul Phusion M/M | |||
** 1.5 ul recT-F | |||
** 1.5 ul recT-R primers | |||
** 1 ul 10 ng/ul S. citri genomic DNA (from Renaudin) | |||
** 46 ul water | |||
* cycle 98/30s (98/15s 55/15s 68/30s)x30 68/10 min | |||
* band visible at 900+ bp (expect 915) | |||
==Reporter design== | |||
* most existing registry reporters have very bad codon usage | |||
* one possible exception is GFPmut3.1, E0040, which has pretty good codon usage but two Sau3AI sites | |||
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Revision as of 21:52, 19 June 2008
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Transformation results
recT gene PCR worked, expected size
Reporter design
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