User:Tk/Notebook/MF-xfm/2008/04/16

From OpenWetWare
< User:Tk‎ | Notebook‎ | MF-xfm‎ | 2008‎ | 04
Revision as of 21:50, 19 June 2008 by Ricardo Vidal (talk | contribs) (→‎Entry title)
Jump to navigationJump to search
MF-xfm <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Transformants

  • One new transformant on the no-DNA no-BSA plate
  • The BSA and DNA transformant from Friday grew cultures in tet medium
  • No new transformants from Monday (none expected)
  • E. coli transformation showed 62 colonies, down from previously
    • probably because I electroporated at 1.5 KV rather than 1.75 KV

New tranformation tests

  • transform testing 4x and 40x concentrations with EPB (no glycerol)
  • three washes at 45 ml each
  • resuspend 50 ul of the 40x concentration in 450 ul for the 4x concentration
  • aliquot 3x 50 ul of low and high concentrations
  • Add 0, 10 ul, 30 ul of 1 M sorbitol as an adjustment to osmolarity
  • Electroporate at 1.5 KV
  • outgrowth for 1 hour at 30C
  • plate 300 ul
  • E. coli transformants done at 1.5 KV and 1.75 KV


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Tk/Notebook/MF-xfm/2008/04/16&oldid=214006"