User:Tk/Notebook/MF-xfm/2008/04/16: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(Autocreate 2008/04/16 Entry for User:Tk/Notebook/MF-xfm) |
|||
| Line 5: | Line 5: | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
== | ==Transformants== | ||
* | * One new transformant on the no-DNA no-BSA plate | ||
* The BSA and DNA transformant from Friday grew cultures in tet medium | |||
* No new transformants from Monday (none expected) | |||
* E. coli transformation showed 62 colonies, down from previously | |||
** probably because I electroporated at 1.5 KV rather than 1.75 KV | |||
==New tranformation tests== | |||
* transform testing 4x and 40x concentrations with EPB (no glycerol) | |||
* three washes at 45 ml each | |||
* resuspend 50 ul of the 40x concentration in 450 ul for the 4x concentration | |||
* aliquot 3x 50 ul of low and high concentrations | |||
* Add 0, 10 ul, 30 ul of 1 M sorbitol as an adjustment to osmolarity | |||
* Electroporate at 1.5 KV | |||
* outgrowth for 1 hour at 30C | |||
* plate 300 ul | |||
* E. coli transformants done at 1.5 KV and 1.75 KV | |||
<!-- ## Do not edit below this line unless you know what you are doing. ## --> | <!-- ## Do not edit below this line unless you know what you are doing. ## --> | ||
|} | |} | ||
Revision as of 21:50, 19 June 2008
 MF-xfm
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Transformants
New tranformation tests
| |
