User:Tk/Notebook/MF-xfm/2008/04/10: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(Autocreate 2008/04/10 Entry for User:Tk/Notebook/MF-xfm) |
|||
Line 5: | Line 5: | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
== | ==Transformations== | ||
* | * Made Electroporation buffer with 12% glycerol for easier freezing and more stable frozen cells | ||
* Transformed some fresh cells | |||
** 45 ml cultures, some very early (1) and some a little late (4) | |||
** wash 2x with 45 ml new EPB | |||
** shake out all liquid from 50 ml tubes | |||
** vortex, leaving about 250-300 ul samples | |||
** electroporated two samples (50 ul + 1 ul transposome), one at 1.25 KV in 1 mm cuvette, another at 1.75 KV | |||
** also did two E. coli controls, same conditions. | |||
** outgrowth in 1700 ul 1161 medium or SOC | |||
** Plate at 1 hour and 1.75 hours (100 ul for E. coli, 300 ul for MF) | |||
** at 1 hour, the growth in #2 was clearly highest, yellow culture medium. #3,4 were also somewhat grown, #1 was not | |||
* Realized that I had been using 1 mm cuvettes for earlier transformations, which may have been the cause of cell death and sparking. | |||
* No sparking this time on any samples. | |||
<!-- ## Do not edit below this line unless you know what you are doing. ## --> | <!-- ## Do not edit below this line unless you know what you are doing. ## --> | ||
|} | |} |
Revision as of 21:46, 19 June 2008
MF-xfm | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Transformations
|