User:Tk/Notebook/MF-xfm/2008/04/07: Difference between revisions
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(Autocreate 2008/04/07 Entry for User:Tk/Notebook/MF-xfm) |
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== | ==To Do== | ||
* | * make more 1161 + tet plates | ||
* make more 1161 medium | |||
* make LB Tet and LB Amp plates | |||
==Transformation results== | |||
* pUC19 10 pg/ul control transformation, plating 0.1 fraction is about 300 colonies | |||
** efficiency is 300*10(fraction)*1e7(10 pg to 1 ug) or 3e10 cfu/ug | |||
* Transposon insertion was 6 colonies, plating 0.1 fraction | |||
** efficiency 60 colonies per 25 ng DNA, or 60*40 cfu/ug = 2.4e3 cfu/ug | |||
** This is 40x below transformation efficiency test of Epicentre | |||
* presumably this is due to the DNA used. Let's try a higher DNA concentration | |||
* After six more hours, 9 colonies showed up. This is 3.6e3 cfu/ug | |||
** 11x less than previous attempt, but 3x less than previous not outgrown 2 hours | |||
** 27x less than Epicentre transformation efficiency QC test | |||
==Web finds== | |||
* [http://bioinformatics.ramapo.edu/QGRS/index.php G-tetrad analysis program] | |||
* [http://mybio.net/biowiki/Sequence_analysis Sequence analysis link web site] | |||
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Revision as of 21:44, 19 June 2008
 MF-xfm
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To Do
Transformation results
Web finds
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