User:Tina Luetke-Eversloh

contact info

Dr. Tina Lütke-Eversloh

University of Rostock
Institute of Biosciences
Microbiology
Albert-Einstein-Str. 3
18051 Rostock
Germany

eMail

research interests

• Development and application of new metabolic engineering tools
• Physiology, genetics and metabolic regulation of anaerobic bacteria
• Genetic engineering of clostridia and other bacteria
• Metabolic engineering of prokaryotes for biofuels, chemicals and polymers

curriculum vitae

UNIVERSITÄT ROSTOCK

• since July 2007: Assistant professor at the Institute of Biosciences, Department of Microbiology
• November 3, 2014: Opening of habilitation process (graduation to "Dr. rer. nat. habil.")
• June 2007: Return grant funded by the Deutsche Forschungsgemeinschaft
  

MASSACHUSETTS INSTITUTE OF TECHNOLOGY, CAMBRIDGE

• June 2004 – May 2007: Postdoc at the Bioinformatics and Metabolic Engineering Laboratory, Department of Chemical Engineering
• June 2004 – May 2006: Research fellowship funded by the Deutsche Forschungsgemeinschaft
  

WESTFÄLISCHE WILHELMS-UNIVERSITÄT, MÜNSTER

• August 2003 – April 2004: Postdoc at the Institute of Molecular Microbiology and Biotechnology
• March 30, 2004: Award of the "VAAM Promotionspreis" (PhD thesis prize, granted by the German Society of General and Applied Microbiology)
• July 22, 2003: PhD graduation (grade: "summa cum laude")
• August 1999 – July 2003: Research assistant at the Institute of Molecular Microbiology and Biotechnology and experimental PhD thesis entitled "Microbial synthesis and biotechnological production of polythioesters"
• June 28, 1999: "Diplom" certificate (grade: "sehr gut")
• May 1998 – June 1999: Experimental diploma thesis entitled "Biochemical and molecular genetic studies on the metabolism of 4-hydroxybutyric acid in Ralstonia eutropha" at the Institute of Microbiology
• October 1993 – June 1999: Studies of Biology
  

STÄDTISCHES GYMNASIUM LAURENTIANUM, ARNSBERG

• June 15, 1993: "Abitur" certificate

publications

ORIGINAL PUBLICATIONS (peer-reviewed)

• Hönicke, D., Lütke-Eversloh, T., Liu, Z., Lehmann, D., Liebl, W. & Ehrenreich, A. (2014). Chemostat cultivation and transcriptional analyses of Clostridium acetobutylicum mutants with defects in the acid and acetone biosynthetic pathways. Appl. Microbiol. Biotechnol. 98, 9777-9794.
• Scheel, M. & Lütke-Eversloh, T. (2013). New options to engineer biofuel microbes: development and application of a high-throughput screening system. Metab. Eng. 17, 51-58.
• Mann, M. S. & Lütke-Eversloh, T. (2013). Thiolase engineering for enhanced butanol production in Clostridium acetobutylicum. Biotechnol. Bioeng. 110, 887-897.
• Lehmann, D., Radomski, N. & Lütke-Eversloh, T. (2012). New insights into the butyric acid metabolism of Clostridium acetobutylicum. Appl. Microbiol. Biotechnol. 96, 1325-1339.
• Mann, M. S., Dragovic, Z., Schirrmacher, G. & Lütke-Eversloh, T. (2012). Over-expression of stress protein encoding genes helps Clostridium acetobutylicum to rapidly adapt to butanol stress. Biotechnol. Lett. 34, 1643-1649.
• Hönicke, D., Janssen, H., Grimmler, C., Ehrenreich, A. & Lütke-Eversloh, T. (2012). Global transcriptional changes of Clostridium acetobutylicum cultures with increased butanol:acetone ratios. N. Biotechnol. 29, 485-493.
• Lehmann, D., Hönicke, D., Ehrenreich, A., Schmidt, M., Weuster-Botz, D., Bahl, H. & Lütke-Eversloh, T. (2012). Modifying the product pattern of Clostridium acetobutylicum: Physiological effects of disrupting the acetate and acetone formation pathways. Appl. Microbiol. Biotechnol. 94, 743-754.
• Lehmann, D. & Lütke-Eversloh, T. (2011). Switching Clostridium acetobutylicum to an ethanol producer by disruption of the butyrate/butanol fermentative pathway. Metab. Eng. 13, 464-473.
• Lütke-Eversloh, T. & Stephanopoulos, G. (2008). Combinatorial pathway analysis for improved L-tyrosine production in Escherichia coli: identification of enzymatic bottlenecks by systematic gene overexpression. Metab. Engin. 10, 69-77.
• Lütke-Eversloh, T. & Stephanopoulos, G. (2007). A semi-quantitative high-throughput screening method for microbial L-tyrosine production in microtiter plates. J. Ind. Microbiol. Biotechnol. 34, 807-811.
• Lütke-Eversloh, T. & Stephanopoulos, G. (2007). L-Tyrosine production by deregulated strains of Escherichia coli. Appl. Microbiol. Biotechnol. 75, 103-110.
• Impallomeni, G., Steinbüchel, A., Lütke-Eversloh, T., Barbuzzi, T. & Ballistreri, A. (2006). Sequencing Microbial Copolymers of 3-Hydroxybutyric and 3-Mercaptoalkanoic Acids by NMR, Electrospray Ionization Mass Spectrometry, and Size Exclusion Chromatography NMR. Biomacromolecules 8, 985-991.
• Wübbeler, J. H., Lütke-Eversloh, T., Vandamme, P. VanTrappen, S. & Steinbüchel, A. (2006). Tetrathiobacter mimigardefordensis sp. nov., isolated from compost, a betaproteobacterium capable of utilizing the organic disulfide 3,3'-dithiodipropionic acid. Int. J. Syst. Evol. Microbiol. 56, 1305-1310.
• Lütke-Eversloh, T. & Stephanopoulos, G. (2005). Feedback inhibition of chorismate mutase/prephenate dehydrogenase (TyrA) of Escherichia coli: generation and characterization of tyrosine-insensitive mutants. Appl. Environ. Microbiol. 71, 7224-7228.
• Kim, D. Y., Lütke-Eversloh, T., Elbanna, K., Thakor, N. & Steinbüchel, A. (2005). Poly(3 mercaptopropionate): A non-biodegradable biopolymer? Biomacromolecules 6, 897-901.
• Thakor, N., Lütke-Eversloh, T. & Steinbüchel, A. (2005). Application of the BPEC pathway for large-scale biotechnological production of poly(3-mercaptopropionate) by recombinant Escherichia coli, including a novel in situ isolation method. Appl. Environ. Microbiol. 71, 835-841.
• Lütke-Eversloh, T., Elbanna, K., Cnockaert, M. C., Mergaert, J., Swings, J., Manaia, C. M. & Steinbüchel, A. (2004). Reclassification of Caenibacterium thermophilum as Schlegelella thermodepolymerans. Int. J. Syst. Evol. Microbiol. 54, 1933-1935.
• Elbanna, K., Lütke-Eversloh, T., Jendrossek, D., Luftmann, H. & Steinbüchel, A. (2004). Studies on the biodegradability of polythioester copolymers and homopolymers by polyhydroxyalkanoate (PHA) degrading bacteria and PHA depolymerases. Arch. Microbiol. 182, 212-225.
• Elbanna, K., Lütke-Eversloh, T., Van Trappen, S., Mergaert, J., Swings, J. & Steinbüchel, A. (2003). Schlegelella thermodepolymerans gen. nov., sp. nov., a novel thermophilic bacterium that degrades poly(3-hydroxybutyrate-co-3-mercaptopropionate). Int. J. Syst. Evol. Microbiol. 53, 1165-1168.
• Lütke-Eversloh, T. & Steinbüchel, A. (2003). Novel precursor substrates for polythioesters (PTE) and limits of PTE biosynthesis in Ralstonia eutropha. FEMS Microbiol. Lett. 221, 191-196.
• Liu, S.-J., Lütke-Eversloh, T. & Steinbüchel, A. (2003). Biosynthesis of poly(3-mercaptopropionate) and poly(3-meraptopropionate-co-3-hydroxybutyrate) with recombinant Escherichia coli. Chin. J. Biotechnol. 19, 195-199.
• Kawada, J., Lütke-Eversloh, T., Steinbüchel, A. & Marchessault, R. H. (2003). Physical properties of microbial polythioesters: Characterization of poly(3-mercaptoalkanoates) synthesized by engineered Escherichia coli. Biomacromolecules 4, 1698-1702.
• Lütke-Eversloh, T., Fischer, A., Remminghorst, U., Kawada, J., Marchessault, R. H., Bögershausen, A., Kalwei, M. Eckert, H., Reichelt, R., Liu, S.-J. & Steinbüchel, A. (2002). Biosynthesis of novel thermoplastic polythioesters by engineered Escherichia coli. Nature Materials 1, 236-240.
• Lütke-Eversloh, T., Kawada, J., Marchessault, R. H. & Steinbüchel, A. (2002). Characterization of biological polythioesters: Physical properties of novel copolymers synthesized by Ralstonia eutropha. Biomacromolecules 3, 159-166.
• Ewering, C., Lütke-Eversloh, T., Luftmann, H. & Steinbüchel, A. (2002). Identification of novel sulfur-containing bacterial polyesters: biosynthesis of poly(3-hydroxy-S-propyl-ω-thioalkanoates) containing thioether linkages in the side chains. Microbiology 148, 1397-1406.
• Lütke-Eversloh, T., Bergander, K., Luftmann, H. & Steinbüchel, A. (2001). Biosynthesis of poly(3-hydroxybutyrate-co-3-mercaptobutyrate) as a sulfur analogue to poly(3-hydroxybutyrate) (PHB). Biomacromolecules 2, 1061-1065.
• Lütke-Eversloh, T., Bergander, K., Luftmann, H. & Steinbüchel, A. (2001). Identification of a new class of biopolymer: bacterial synthesis of a sulfur containing polymer with thioester linkages. Microbiology 147, 11-19.
• Lütke-Eversloh, T. & Steinbüchel, A. (1999). Biochemical and molecular characterization of a succinate semialdehyde dehydrogenase involved in the catabolism of 4-hydroxybutyric acid in Ralstonia eutropha. FEMS Microbiol. Lett. 181, 63-71.
  

REVIEW ARTICLES (peer-reviewed)

• Lütke-Eversloh, T. (2014). Application of new metabolic engineering tools for Clostridium acetobutylicum. Appl. Microbiol. Biotechnol. 98, 5823-5837.
• Lütke-Eversloh, T. & Bahl, H. (2011). Metabolic engineering of Clostridium acetobutylicum: Recent advances to improve butanol production. Curr. Opin. Biotechnol. 22, 634-647.
• Lütke-Eversloh, T., Santos, C. N. & Stephanopoulos, G. (2007). Perspectives of biotechnological production of L-tyrosine and its applications. Appl. Microbiol. Biotechnol. 77, 751-762.
• Lütke-Eversloh, T. & Steinbüchel, A. (2004). Microbial polythioesters. Macromol. Biosci. 4, 165-174.
• Steinbüchel, A. & Lütke-Eversloh, T. (2003). Metabolic engineering and pathway construction for biotechnological production of relevant polyhydroxyalkanoates in microorganisms. Biochem. Engin. J. 16, 81-96.
  

INTERNATIONAL PATENTS

• Dragovic, Z., Schirrmacher, G., Lütke Eversloh, T., Mann, M. S., Schmidt, M., & Weuster-Botz, D. (2013). Improvement of clostridial butanol production by gene overexpression. EP 13158012.8.
• Dragovic, Z., Schirrmacher, G., Lütke-Eversloh, T. & Mann, M. S. (2012). Engineered Clostridium strain for butanol production. WO 2014/006203.
• Lütke-Eversloh, T. & Stephanopoulos, G. N. (2005). Mutations for enhanced tyrosine production. WO 2006/124895.
• Lütke-Eversloh, T., Steinbüchel, A. & Ewering, C. (2000). Sulfur containing polyhydroxyalkanoate compositions and method of production. WO 2002/16627.

links

• Publications @ PubMed [1]
• Publications @ Scopus [2]
• Mikrobiologie Rostock [3]
• Stephanopoulos Lab [4]
• IMMB Münster [5]