User:Tim Henry/Notebook/D1S80/2012/02/24
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D1S80 PCRLooking at datasheet for Maxime i-Taq, it suggest using 10pM of primer. From primer datasheet it appears it is 200mL, 100μM - if I have this correct it should be 0.5μM/1μL. This would correspond to protocols for other taq polymerases (0.1-1μM suggested). To dilute, 49μL dH2O + 1μL. Then use 1μL (10pM) 1.5 μL template (usually use 2.0) 1μL primer each 16.5μL dH2O 30 cycles of denature/anneal/extension
Put template + primers on 94°C first before adding taq, pseudo hot start style.
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