User:Tim Henry/Notebook/D1S80/2012/02/08

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D1S80

1.1% agarose gel - TAE buffer

1.1 chosen by optimal gel calculator (online) for ~575bp product.

75mL TAE + .825 agarose

70v. Waited until BB (purple ~500bp) was past half way.

Product only present for T and G lanes

T confirmed heterozygous

G homozygous or close doublet/heterozygous


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