User:Tara K. Luckau/Notebook/Team ConGen/Entry Base: Difference between revisions

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* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
===Fragment Analysis Submission===
:: [[Image:20130226_Frag.png|900 px]]
* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151778 801124151778]
:: [[Image:20130226_FedEx.png|700 px]]
* UAGC Submission# PPTX
* FedEx picked up 2:44pm, 26 February
* FedEx delivered 9:29am, 27 February
* UAGC received 10:58pm, 27 February
* UAGC completed 10:41am, 28 February





Revision as of 16:02, 12 March 2013

Tara's Lab Notebook <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page


CNTI Acos5

PCR


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


  • mispriming everywhere - primer pair not usable for CNHY
  • lots of mispriming, but may be able to use conditions indicated by orange face (), if needed, under stringent conditions
  • favorable conditions indicated by


Fragment Analysis Submission



  • UAGC Submission# PPTX


  • FedEx picked up 2:44pm, 26 February
  • FedEx delivered 9:29am, 27 February
  • UAGC received 10:58pm, 27 February
  • UAGC completed 10:41am, 28 February