User:Tara K. Luckau/Notebook/Team ConGen/Entry Base: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
No edit summary
Line 9: Line 9:




==CNHY Acos5==
==CNTI Acos5==




===PCR===
===PCR===
* [[Image:20111023_PCRa.png|800 px]]
* [[Image:20120110_PCRa.png|800 px]]




Line 28: Line 28:




[[Image:20111023_Gel.png|800 px]]
[[Image:20120110_Gela.png|800 px]]


* mispriming everywhere - primer pair not usable for CNHY
* mispriming everywhere - primer pair not usable for CNHY

Revision as of 10:01, 10 January 2012

Tara's Lab Notebook <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page


CNTI Acos5

PCR


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


  • mispriming everywhere - primer pair not usable for CNHY
  • lots of mispriming, but may be able to use conditions indicated by orange face (), if needed, under stringent conditions
  • favorable conditions indicated by




Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Tara_K._Luckau/Notebook/Team_ConGen/Entry_Base&oldid=575303"