User:Tara K. Luckau/Notebook/Team ConGen/Entry Base: Difference between revisions
From OpenWetWare
No edit summary |
No edit summary |
||
Line 6: | Line 6: | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
__TOC__ | |||
==27 September 2010 - Ordered Primers== | ==27 September 2010 - Ordered Primers== | ||
* Ordered first set of primers | * Ordered first set of primers | ||
Line 17: | Line 18: | ||
# F M13 - R | # F M13 - R | ||
# F M13 - R pigtail | # F M13 - R pigtail | ||
* If they don't hinder efforts | * If they don't hinder efforts or change optimization decisions, then may make optimization of multiplexes cheaper! | ||
==11 October 2010 - PCR Thermalcycler Testing== | ==11 October 2010 - PCR Thermalcycler Testing== | ||
===Purpose=== | |||
* 10μL in each well of full-skirt PCR plate, PCR seal | |||
* First set-up of thermalcycler, established User, Protocols, Plates and Masters | |||
* Data: 20101011_TKL_CyclerTest.tad | |||
===Results=== | |||
* Evaporation all around edges | |||
* Try using silicone mat? | |||
===Purpose=== | |||
* 10μL in each well of full-skirt PCR plate, PCR seal, 1 silicone mat | |||
* Data: 20101011_TKL_CyclerTest1Mat.tad | |||
===Results=== | |||
* Still evaporation around edges, but better | |||
* Try using 2 silicone mats? | |||
==12 October 2010 - PCR Thermalcycler Testing== | |||
===Purpose=== | |||
* 10μL in each well of full-skirt PCR plate, PCR seal, 2 silicone mats | |||
* Data: 20101012_TKL_CyclerTest2Mat.tad | |||
===Results=== | |||
* No evaporation! | |||
* Silicone mats got stuck on lid | |||
* Try using 2 silicone mats with tape? | |||
* Try getting samples of different brand of silicone mats, different shape, no holes? | |||
==18 October 2010 - PCR Thermalcycler Testing== | |||
===Purpose=== | |||
* 10µL in each well of full-skirt PCR plate, PCR seal, 2 silicone mats, lab tape on opposite sides | |||
* Data: 20102018_TKL_CyclerTest2MatTape.tad | |||
===Results=== | |||
* H6, H7 evaporated, but I think it's workable | |||
* Plan to do first optimization PCR tomorrow! | |||
==18 October 2010 - Primer Rehydration, Scun2 | |||
===Purpose=== | |||
* Rehydrate Scun2 primers for testing tomorrow | |||
# Scun2-F | |||
# Scun2-R | |||
# Scun2-F-M13F(-21) | |||
# Scun2-R-pigtail | |||
===Calculations=== | |||
* | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Revision as of 12:23, 25 October 2010
Tara's Lab Notebook | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page |
27 September 2010 - Ordered Primers
11 October 2010 - PCR Thermalcycler TestingPurpose
Results
Purpose
Results
12 October 2010 - PCR Thermalcycler TestingPurpose
Results
18 October 2010 - PCR Thermalcycler TestingPurpose
Results
==18 October 2010 - Primer Rehydration, Scun2 Purpose
Calculations |