User:Tara K. Luckau/Notebook/Team ConGen/2013/06/06

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(Autocreate 2013/06/06 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
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==CNTI Acos5==
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==SCOC MP2 Plates1-4==
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* comments
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* Plates 1-4 (not batches, to differentiate between SCOC MP1 and SCOC MP2)
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:: [[Image:20130110_PCRa.png|800 px]]
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:: [[Image:20130606_PCRa1.png|800 px]]
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:: [[Image:20130606_PCRa2.png|800 px]]
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===Gel===
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==ASHY MP1/2/3 Opt==
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{| {{table}} style="text-align: center"
 
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|-
 
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! Pour !! Load !! Run
 
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|-
 
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| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
 
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|-
 
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| 27 µL GelRed || 6 µL ladder || 45 minutes
 
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|}
 
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===PCR===
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:: [[Image:20130110_Gela.png|800 px]]
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* mispriming everywhere - primer pair not usable for CNHY
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* Re-do from 18Mar2013 (everything failed unexpectedly)
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* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
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:: [[Image:20130606_PCRb.png|800 px]]
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* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
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* UAGC Submission# ????
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* UAGC Submission# BPSH, HBDA, PMGS, GHDY, QSNF
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:: [[Image:20130227_Frag.png|900 px]]
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:: [[Image:20130606_Fraga.png|900 px]]
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:: [[Image:20130606_Fragb.png|900 px]]
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* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151779 801124151779]
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* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124713100 801124713100]
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:: [[Image:20130227_FedEx.png|700 px]]
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:: [[Image:20130606_FedEx.png|700 px]]

Revision as of 17:49, 9 June 2013

Tara's Lab Notebook Main project page
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Contents


SCOC MP2 Plates1-4

PCR

  • Plates 1-4 (not batches, to differentiate between SCOC MP1 and SCOC MP2)


ASHY MP1/2/3 Opt

PCR

  • Re-do from 18Mar2013 (everything failed unexpectedly)


Fragment Analysis Submission

  • UAGC Submission# BPSH, HBDA, PMGS, GHDY, QSNF


Image:20130606 FedEx.png


  • FedEx picked up ____pm, 26 February
  • FedEx delivered ____am, 27 February
  • UAGC received ____am, 27 February
  • UAGC completed ____am, 28 February




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