User:Tara K. Luckau/Notebook/Team ConGen/2013/05/22: Difference between revisions

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(Autocreate 2013/05/22 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
 
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==CNTI Acos5==
==SCOC MP2==




===PCR===
===PCR===
* [[Image:20130110_PCRa.png|800 px]]
* [[Image:20130522_PCR.png|800 px]]
 
 
===Gel===
 
{| {{table}} style="text-align: center"
|-
! Pour !! Load !! Run
|-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
|-
| 27 µL GelRed || 6 µL ladder || 45 minutes
|}
 
 
[[Image:20130110_Gela.png|800 px]]
 
* mispriming everywhere - primer pair not usable for CNHY
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]




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:: [[Image:20130227_Frag.png|900 px]]
:: [[Image:20130522_Frag.png|900 px]]




* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151779 801124151779]
* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124713122 801124713122]
:: [[Image:20130227_FedEx.png|700 px]]
:: [[Image:20130522_FedEx.png|700 px]]




* UAGC Submission# ????
* UAGC Submission# HBXH




Revision as of 09:02, 23 May 2013

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SCOC MP2

PCR


Fragment Analysis Submission



  • UAGC Submission# HBXH


  • FedEx picked up ____pm, 26 February
  • FedEx delivered ____am, 27 February
  • UAGC received ____am, 27 February
  • UAGC completed ____am, 28 February




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