User:Tara K. Luckau/Notebook/Team ConGen/2013/05/22: Difference between revisions

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===PCR===
===PCR===
* [[Image:20130522_PCR.png|800 px]]
 
 
* From last two PCRs, looks like not enough template DNA (controls are strong, scorable; some samples had low amplification that seem would be fine with additional template)
* SCOC MP2 Plate5 samples plated in triplicate (plus 5 additional samples from various extraction batches), 2µL then 4µL then 6µL. Dessicated overnight as usual.
:: [[Image:20130522_PCR.png|800 px]]
* Complications:
** I derped - forgot to actually put the plate in the cycler (left it in the centrifuge) for the duration of the cycling. Noticed when I went to remove the plate from the cycler, only to find the cycler was empty. Plate was sitting in centrifuge for approx. 1.5 hours.




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* UAGC Submission# HBXH
:: [[Image:20130522_Frag.png|900 px]]
:: [[Image:20130522_Frag.png|900 px]]


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* UAGC Submission# HBXH
* FedEx picked up 4:45pm, 22 May
 
* FedEx delivered 9:37am, 23 May
 
* UAGC received 11:27am, 23 May
* FedEx picked up ____pm, 26 February
* UAGC completed 10:33am, 24 May
* FedEx delivered ____am, 27 February
* UAGC received ____am, 27 February
* UAGC completed ____am, 28 February




Revision as of 14:33, 9 June 2013

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SCOC MP2

PCR

  • From last two PCRs, looks like not enough template DNA (controls are strong, scorable; some samples had low amplification that seem would be fine with additional template)
  • SCOC MP2 Plate5 samples plated in triplicate (plus 5 additional samples from various extraction batches), 2µL then 4µL then 6µL. Dessicated overnight as usual.
  • Complications:
    • I derped - forgot to actually put the plate in the cycler (left it in the centrifuge) for the duration of the cycling. Noticed when I went to remove the plate from the cycler, only to find the cycler was empty. Plate was sitting in centrifuge for approx. 1.5 hours.


Fragment Analysis Submission

  • UAGC Submission# HBXH



  • FedEx picked up 4:45pm, 22 May
  • FedEx delivered 9:37am, 23 May
  • UAGC received 11:27am, 23 May
  • UAGC completed 10:33am, 24 May




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