User:Tara K. Luckau/Notebook/Team ConGen/2013/03/18

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(Autocreate 2013/03/18 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
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==CNTI Acos5==
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==SCOC MP2 Opt==
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* comments
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* primer concentration gradient for SW614-A4 because low-intensity marker
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:: [[Image:20130110_PCRa.png|800 px]]
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:: [[Image:20130318_PCRa.png|800 px]]
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===Gel===
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==ASHY MP1/2/3 Opt==
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{| {{table}} style="text-align: center"
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|-
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===PCR===
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! Pour !! Load !! Run
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|-
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| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
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|-
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| 27 µL GelRed || 6 µL ladder || 45 minutes
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|}
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:: [[Image:20130110_Gela.png|800 px]]
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* for MP1, removed Ai5043 because low-intensity, moved Ai5013 from NED to FAM
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* for MP2, removed Ai5057 because low-intensity, may have used Buffer D instead of F in last run
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* for MP3, new multiplex from MP1's and MP2's rejects (Ai5043 and Ai5057) + Cvanµ24
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:: [[Image:20130318_PCRb.png|800 px]]
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* mispriming everywhere - primer pair not usable for CNHY
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==Gel==
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* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
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* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
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===Fragment Analysis Submission===
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:: [[Image:20130319_Gel.png|800 px]]
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* UAGC Submission# ????
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==Fragment Analysis Submission==
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:: [[Image:20130227_Frag.png|900 px]]
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* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151779 801124151779]
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* UAGC Submission# MDQQ
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:: [[Image:20130227_FedEx.png|700 px]]
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:: [[Image:20130319_Fraga.png|900 px]]
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:: [[Image:20130319_Fragb.png|900 px]]
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* FedEx picked up ____pm, 26 February
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* UAGC received 3:06pm, 20 March
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* FedEx delivered ____am, 27 February
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* UAGC completed 10:23am, 21 March
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* UAGC received ____am, 27 February
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* UAGC completed ____am, 28 February
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Revision as of 18:19, 9 June 2013

Tara's Lab Notebook Main project page
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Contents


SCOC MP2 Opt

PCR

  • primer concentration gradient for SW614-A4 because low-intensity marker


ASHY MP1/2/3 Opt

PCR

  • for MP1, removed Ai5043 because low-intensity, moved Ai5013 from NED to FAM
  • for MP2, removed Ai5057 because low-intensity, may have used Buffer D instead of F in last run
  • for MP3, new multiplex from MP1's and MP2's rejects (Ai5043 and Ai5057) + Cvanµ24


Gel


Fragment Analysis Submission

  • UAGC Submission# MDQQ
Image:20130319 Fraga.png
Image:20130319 Fragb.png


  • UAGC received 3:06pm, 20 March
  • UAGC completed 10:23am, 21 March




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