User:Tara K. Luckau/Notebook/Team ConGen/2013/03/18: Difference between revisions

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==CNTI Acos5==
==SCOC MP2 Opt==




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* comments
* primer concentration gradient for SW614-A4 because low-intensity marker
:: [[Image:20130110_PCRa.png|800 px]]
:: [[Image:20130318_PCRa.png|800 px]]




===Gel===
==ASHY MP1/2/3 Opt==


{| {{table}} style="text-align: center"
 
|-
===PCR===
! Pour !! Load !! Run
 
|-
 
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
* for MP1, removed Ai5043 because low-intensity, moved Ai5013 from NED to FAM
|-
* for MP2, removed Ai5057 because low-intensity, may have used Buffer D instead of F in last run
| 27 µL GelRed || 6 µL ladder || 45 minutes
* for MP3, new multiplex from MP1's and MP2's rejects (Ai5043 and Ai5057) + Cvanµ24
|}
:: [[Image:20130318_PCRb.png|800 px]]




:: [[Image:20130110_Gela.png|800 px]]
==Gel==




* mispriming everywhere - primer pair not usable for CNHY
:: [[Image:20130319_Gel.png|800 px]]
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* Gel was run with Shannon's samples
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
* I don't remember which lanes were which; mine are probably the four to the right of the ladder




===Fragment Analysis Submission===
==Fragment Analysis Submission==




* UAGC Submission# ????
* submitted/mailed with Shannon's stuff; no FedEx information
:: [[Image:20130227_Frag.png|900 px]]




* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151779 801124151779]
* UAGC Submission# MDQQ
:: [[Image:20130227_FedEx.png|700 px]]
:: [[Image:20130319_Frag.png|900 px]]




* FedEx picked up ____pm, 26 February
* UAGC received 3:06pm, 20 March
* FedEx delivered ____am, 27 February
* UAGC completed 10:23am, 21 March
* UAGC received ____am, 27 February
* UAGC completed ____am, 28 February




Revision as of 15:29, 9 June 2013

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SCOC MP2 Opt

PCR

  • primer concentration gradient for SW614-A4 because low-intensity marker


ASHY MP1/2/3 Opt

PCR

  • for MP1, removed Ai5043 because low-intensity, moved Ai5013 from NED to FAM
  • for MP2, removed Ai5057 because low-intensity, may have used Buffer D instead of F in last run
  • for MP3, new multiplex from MP1's and MP2's rejects (Ai5043 and Ai5057) + Cvanµ24


Gel

  • Gel was run with Shannon's samples
  • I don't remember which lanes were which; mine are probably the four to the right of the ladder


Fragment Analysis Submission

  • submitted/mailed with Shannon's stuff; no FedEx information


  • UAGC Submission# MDQQ


  • UAGC received 3:06pm, 20 March
  • UAGC completed 10:23am, 21 March




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