User:Tara K. Luckau/Notebook/Team ConGen/2013/02/27: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
No edit summary
Line 20: Line 20:


* Frag data: PPTX
* Frag data: PPTX
* also using frag data from FHVZ (late November 2012)
** (apparently, I already did this PCR of uniplexing each SW marker, then throwing it all in together ... oops)




Line 26: Line 28:
! Marker !! SYR Genotype !! TP3 Genotype !! Uniplex comments !! Multiplex comments
! Marker !! SYR Genotype !! TP3 Genotype !! Uniplex comments !! Multiplex comments
|-
|-
| style="background: yellow" | SW614-A1 (NED) || 410 415 || [poop] || tall @ 8000, very clean || short @ 300, clean
| style="background: yellow" | SW614-A1 (NED) || 410 415 || style="background: Silver" | [poop] || tall @ 8000, very clean || short @ 300, clean
|-
|-
| style="background: royalblue" | SW614-A4 (FAM) || 395 396 || 396 398 || tall @ 15000 || short @ 300
| style="background: royalblue" | '''SW614-A4 (FAM)''' || '''395 396''' || '''396 398''' || '''tall @ 15000''' || '''short @ 300'''
|-
|-
| style="background: red" | SW614-A6 (PET) || [poop] || 255 275 || tall @ 8000 || good @ 1500
| style="background: red" | SW614-A6 (PET) || style="background: Silver" | [poop] || 255 275 || tall @ 8000 || good @ 1500
|-
|-
| style="background: green" | SW614-A7 (HEX) || 252 252 || 248 248 || tall @ 14000 || no amplification
| style="background: green" | SW614-A7 (HEX) || 252 252 || 248 248 || tall @ 14000 || style="background: Silver" | no amplification
|-
|-
| style="background: green" | SW614-B1 (HEX) || 316 316 || 299 316 || tall @ 16000, one replicate of SYR poop || good @ 1200
| style="background: green" | '''SW614-B1 (HEX)''' || '''316 316''' || '''299 316''' || '''tall @ 16000, one replicate of SYR poop''' || '''good @ 1200'''
|-
|-
| style="background: royalblue" | SW614-B6 (FAM) || 308 310 || 308 310 || tall @ 11000 || no amplification
| style="background: royalblue" | SW614-B6 (FAM) || 308 310 || 308 310 || tall @ 11000 || style="background: Silver" | no amplification
|-
|-
| style="background: royalblue" | SW614-B10 (FAM) || 188 194 || 186 198 || tall @ 12000, one replicate of SYR violates slope rule || no amplification
| style="background: royalblue" | SW614-B10 (FAM) || 188 194 || 186 198 || tall @ 12000, one replicate of SYR violates slope rule || style="background: Silver" | no amplification
|-
|-
| style="background: yellow" | SW614-B12 (NED) || 285 285 || 255 274 || tall @ 14000 || good @ 2000
| style="background: yellow" | '''SW614-B12 (NED)''' || '''285 285''' || '''255 274''' || '''tall @ 14000''' || '''good @ 2000'''
|-
|-
|}
|}

Revision as of 12:46, 6 March 2013

Tara's Lab Notebook <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


SCOC MP2 Optimization

  • uniplex SW markers to see quality of peaks and range of alleles
  • multiplex SW markers as first glance of how multiplex behaves


Results

  • Frag data: PPTX
  • also using frag data from FHVZ (late November 2012)
    • (apparently, I already did this PCR of uniplexing each SW marker, then throwing it all in together ... oops)


Marker SYR Genotype TP3 Genotype Uniplex comments Multiplex comments
SW614-A1 (NED) 410 415 [poop] tall @ 8000, very clean short @ 300, clean
SW614-A4 (FAM) 395 396 396 398 tall @ 15000 short @ 300
SW614-A6 (PET) [poop] 255 275 tall @ 8000 good @ 1500
SW614-A7 (HEX) 252 252 248 248 tall @ 14000 no amplification
SW614-B1 (HEX) 316 316 299 316 tall @ 16000, one replicate of SYR poop good @ 1200
SW614-B6 (FAM) 308 310 308 310 tall @ 11000 no amplification
SW614-B10 (FAM) 188 194 186 198 tall @ 12000, one replicate of SYR violates slope rule no amplification
SW614-B12 (NED) 285 285 255 274 tall @ 14000 good @ 2000



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Tara_K._Luckau/Notebook/Team_ConGen/2013/02/27&oldid=681263"