User:Tara K. Luckau/Notebook/Team ConGen/2013/02/26

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Autocreate 2013/02/26 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
Line 9: Line 9:
-
==CNTI Acos5==
+
==SCOC MP2 Optimization==
-
===PCR===
+
* uniplex SW markers to see quality of peaks and range of alleles
-
* [[Image:20120110_PCRa.png|800 px]]
+
* multiplex SW markers as first glance of how multiplex behaves
===Gel===
===Gel===
-
{| {{table}} style="text-align: center"
 
-
|-
 
-
! Pour !! Load !! Run
 
-
|-
 
-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
 
-
|-
 
-
| 27 µL GelRed || 6 µL ladder || 45 minutes
 
-
|}
 
 +
[[Image:20130226_Gel.png|800 px]]
-
[[Image:20120110_Gela.png|800 px]]
+
* stuff
-
 
+
* stuff
-
* mispriming everywhere - primer pair not usable for CNHY
+
-
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
+
-
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
+

Revision as of 16:29, 26 February 2013

Tara's Lab Notebook Main project page
Previous entry      Next entry

Contents


SCOC MP2 Optimization

  • uniplex SW markers to see quality of peaks and range of alleles
  • multiplex SW markers as first glance of how multiplex behaves


Gel

  • stuff
  • stuff




Retrieved from "http://openwetware.org/wiki/User:Tara_K._Luckau/Notebook/Team_ConGen/2013/02/26"
Personal tools