User:Tara K. Luckau/Notebook/Team ConGen/2013/02/26

Current revision

Tara's Lab Notebook

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-==CNTI Acos5==
+==SCOC MP2 Optimization==
-===PCR===
+* uniplex SW markers to see quality of peaks and range of alleles
-* [[Image:20120110_PCRa.png|800 px]]
+* multiplex SW markers as first glance of how multiplex behaves
 ===Gel===
-{| {{table}} style="text-align: center"
-|-
-! Pour !! Load !! Run
-|-
-| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
-|-
-| 27 µL GelRed || 6 µL ladder || 45 minutes
-|}
+[[Image:20130226_Gel.png|800 px]]
-[[Image:20120110_Gela.png|800 px]]
-* mispriming everywhere - primer pair not usable for CNHY
+* gel floated sideways, so it looks funny, but whatevs
-* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
+* clean bands in uniplexes
-* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
+* multiplex has amplification
+* send to frag!
+===Fragment Analysis Submission===
+:: [[Image:20130226_Frag.png|900 px]]
+* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151778 801124151778]
+:: [[Image:20130226_FedEx.png|700 px]]
+* UAGC Submission# PPTX
+* FedEx picked up 2:44pm, 26 February
+* FedEx delivered 9:29am, 27 February
+* UAGC received 10:58pm, 27 February
+* UAGC completed 10:41am, 28 February