User:Tara K. Luckau/Notebook/Team ConGen/2013/02/25: Difference between revisions

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==CNTI Acos5==
==SCOC MP1 Batch 2 Plate 1&2 (re-do)==




===PCR===
===Fragment Analysis Submission===
* [[Image:20120110_PCRa.png|800 px]]
 
 
* combined all PCR product into MP1-G plate (approximately 10µL MP1-B + 10µL MP1-G + 10µL MP1-Y)
 
 
:: [[Image:20130225_Frag.png|900 px]]
 
 
* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151675 801124151675]
:: [[Image:20130225_FedEx.png|700 px]]
 
 
* UAGC Submission# XPTR & VMZX
 
 
* FedEx picked up 2:41pm, 25 February
* FedEx delivered 9:12am, 26 February
* UAGC received 10:43am, 26 February
* UAGC completed 9:28am, 27 February
 
 
==SCOC MP2 Optimization==
 


* uniplex SW markers to see quality of peaks and range of alleles
* multiplex SW markers as first glance of how multiplex behaves


===Gel===


{| {{table}} style="text-align: center"
===PCR===
|-
! Pour !! Load !! Run
|-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
|-
| 27 µL GelRed || 6 µL ladder || 45 minutes
|}




[[Image:20120110_Gela.png|800 px]]
* [[Image:20130225_PCRa.png|800 px]]


* mispriming everywhere - primer pair not usable for CNHY
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]


* [[Image:20130225_PCRb.png|800 px]]




Revision as of 14:59, 27 February 2013

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SCOC MP1 Batch 2 Plate 1&2 (re-do)

Fragment Analysis Submission

  • combined all PCR product into MP1-G plate (approximately 10µL MP1-B + 10µL MP1-G + 10µL MP1-Y)




  • UAGC Submission# XPTR & VMZX


  • FedEx picked up 2:41pm, 25 February
  • FedEx delivered 9:12am, 26 February
  • UAGC received 10:43am, 26 February
  • UAGC completed 9:28am, 27 February


SCOC MP2 Optimization

  • uniplex SW markers to see quality of peaks and range of alleles
  • multiplex SW markers as first glance of how multiplex behaves


PCR




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