User:Tara K. Luckau/Notebook/Team ConGen/2013/02/25

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(Autocreate 2013/02/25 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
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==CNTI Acos5==
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==SCOC MP2 Optimization==
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===PCR===
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* uniplex SW markers to see quality of peaks and range of alleles
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* [[Image:20120110_PCRa.png|800 px]]
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* multiplex SW markers as first glance of how multiplex behaves
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===Gel===
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===PCR===
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{| {{table}} style="text-align: center"
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|-
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! Pour !! Load !! Run
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|-
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| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
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|-
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| 27 µL GelRed || 6 µL ladder || 45 minutes
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|}
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[[Image:20120110_Gela.png|800 px]]
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* [[Image:20130225_PCRa.png|800 px]]
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* mispriming everywhere - primer pair not usable for CNHY
 
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* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
 
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* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
 
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* [[Image:20130225_PCRb.png|800 px]]

Revision as of 15:28, 26 February 2013

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Contents


SCOC MP2 Optimization

  • uniplex SW markers to see quality of peaks and range of alleles
  • multiplex SW markers as first glance of how multiplex behaves


PCR




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