User:Tara K. Luckau/Notebook/Team ConGen/2013/02/25

Current revision

Tara's Lab Notebook

combined all PCR product into MP1-G plate (approximately 10µL MP1-B + 10µL MP1-G + 10µL MP1-Y)

@@ Line 9: / Line 9: @@
-==CNTI Acos5==
+==SCOC MP1 Batch 2 Plate 1&2 (re-do)==
-===PCR===
+===Fragment Analysis Submission===
-* [[Image:20120110_PCRa.png|800 px]]
-===Gel===
+* combined all PCR product into MP1-G plate (approximately 10µL MP1-B + 10µL MP1-G + 10µL MP1-Y)
-{| {{table}} style="text-align: center"
-|-
+:: [[Image:20130225_Frag.png|900 px]]
-! Pour !! Load !! Run
-|-
-| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
+* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151675 801124151675]
-|-
+:: [[Image:20130225_FedEx.png|700 px]]
-| 27 µL GelRed || 6 µL ladder || 45 minutes
-|}
+* UAGC Submission# XPTR & VMZX
+* FedEx picked up 2:41pm, 25 February
+* FedEx delivered 9:12am, 26 February
+* UAGC received 10:43am, 26 February
+* UAGC completed 9:28am, 27 February
+==SCOC MP2 Optimization==
+* uniplex SW markers to see quality of peaks and range of alleles
+* multiplex SW markers as first glance of how multiplex behaves
+===PCR===
-[[Image:20120110_Gela.png|800 px]]
+* [[Image:20130225_PCRa.png|800 px]]
-* mispriming everywhere - primer pair not usable for CNHY
-* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
-* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
+* [[Image:20130225_PCRb.png|800 px]]