User:Tara K. Luckau/Notebook/Team ConGen/2013/02/25

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(Autocreate 2013/02/25 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
Current revision (17:59, 27 February 2013) (view source)
 
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==CNTI Acos5==
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==SCOC MP1 Batch 2 Plate 1&2 (re-do)==
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===PCR===
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===Fragment Analysis Submission===
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* [[Image:20120110_PCRa.png|800 px]]
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===Gel===
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* combined all PCR product into MP1-G plate (approximately 10µL MP1-B + 10µL MP1-G + 10µL MP1-Y)
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{| {{table}} style="text-align: center"
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|-
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:: [[Image:20130225_Frag.png|900 px]]
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! Pour !! Load !! Run
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|-
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| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
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* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151675 801124151675]
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|-
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:: [[Image:20130225_FedEx.png|700 px]]
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| 27 µL GelRed || 6 µL ladder || 45 minutes
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|}
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* UAGC Submission# XPTR & VMZX
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* FedEx picked up 2:41pm, 25 February
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* FedEx delivered 9:12am, 26 February
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* UAGC received 10:43am, 26 February
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* UAGC completed 9:28am, 27 February
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==SCOC MP2 Optimization==
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* uniplex SW markers to see quality of peaks and range of alleles
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* multiplex SW markers as first glance of how multiplex behaves
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===PCR===
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[[Image:20120110_Gela.png|800 px]]
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* [[Image:20130225_PCRa.png|800 px]]
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* mispriming everywhere - primer pair not usable for CNHY
 
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* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
 
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* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
 
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* [[Image:20130225_PCRb.png|800 px]]

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Contents


SCOC MP1 Batch 2 Plate 1&2 (re-do)

Fragment Analysis Submission

  • combined all PCR product into MP1-G plate (approximately 10µL MP1-B + 10µL MP1-G + 10µL MP1-Y)




  • UAGC Submission# XPTR & VMZX


  • FedEx picked up 2:41pm, 25 February
  • FedEx delivered 9:12am, 26 February
  • UAGC received 10:43am, 26 February
  • UAGC completed 9:28am, 27 February


SCOC MP2 Optimization

  • uniplex SW markers to see quality of peaks and range of alleles
  • multiplex SW markers as first glance of how multiplex behaves


PCR




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