User:Tara K. Luckau/Notebook/Team ConGen/2013/02/21: Difference between revisions

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==CNTI Acos5==
==re-do SCOC MP1 Batch 2==




===PCR===
===PCR===
* [[Image:20120110_PCRa.png|800 px]]
* MP1-G
* [[Image:20130221_PCRg.png|800 px]]




===Gel===
* MP1-Y
 
* [[Image:20130221_PCRy.png|800 px]]
{| {{table}} style="text-align: center"
|-
! Pour !! Load !! Run
|-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
|-
| 27 µL GelRed || 6 µL ladder || 45 minutes
|}
 
 
[[Image:20120110_Gela.png|800 px]]
 
* mispriming everywhere - primer pair not usable for CNHY
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]




* [[Image:20130220_PCRPlate.png|800 px]]




Revision as of 14:49, 25 February 2013

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re-do SCOC MP1 Batch 2

PCR

  • MP1-G


  • MP1-Y




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