User:Tara K. Luckau/Notebook/Team ConGen/2013/02/09

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search

Tara K. Luckau (Talk | contribs)
(Autocreate 2013/02/09 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
Next diff →

Revision as of 17:12, 12 February 2013

Tara's Lab Notebook Main project page
Previous entry      Next entry

Contents


CNTI Acos5

PCR


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


  • mispriming everywhere - primer pair not usable for CNHY
  • lots of mispriming, but may be able to use conditions indicated by orange face (), if needed, under stringent conditions
  • favorable conditions indicated by




Retrieved from "http://openwetware.org/wiki/User:Tara_K._Luckau/Notebook/Team_ConGen/2013/02/09"
Personal tools