User:Tara K. Luckau/Notebook/Team ConGen/2013/02/09: Difference between revisions

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==CNTI Cvanµ23==
==ASHY Cvanµ23==




Line 26: Line 26:
[[Image:20130209_Gela.png|800 px]]
[[Image:20130209_Gela.png|800 px]]


* mispriming everywhere - primer pair not usable for CNHY
* mispriming everywhere - primer pair not usable for ASHY
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]




==CNTI Cvanµ24==
==ASHY Cvanµ24==




Line 52: Line 50:
[[Image:20130209_Gelb.png|800 px]]
[[Image:20130209_Gelb.png|800 px]]


* mispriming everywhere - primer pair not usable for CNHY
* it works! Yay! Many possibilities
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]




==CNTI Cvanµ26==
==ASHY Cvanµ26==




Line 78: Line 74:
[[Image:20130209_Gelc.png|800 px]]
[[Image:20130209_Gelc.png|800 px]]


* mispriming everywhere - primer pair not usable for CNHY
* mispriming everywhere - primer pair not usable for ASHY
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]




==CNTI Cvanµ27==
==ASHY Cvanµ27==




Line 104: Line 98:
[[Image:20130209_Geld.png|800 px]]
[[Image:20130209_Geld.png|800 px]]


* mispriming everywhere - primer pair not usable for CNHY
* mispriming everywhere - primer pair not usable for ASHY
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]




Revision as of 14:43, 12 February 2013

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ASHY Cvanµ23

Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


  • mispriming everywhere - primer pair not usable for ASHY


ASHY Cvanµ24

PCR


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


  • it works! Yay! Many possibilities


ASHY Cvanµ26

PCR


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


  • mispriming everywhere - primer pair not usable for ASHY


ASHY Cvanµ27

PCR


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


  • mispriming everywhere - primer pair not usable for ASHY




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