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 Tara's Lab Notebook
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CNTI Cvanµ23
Gel
| Pour |
Load |
Run
|
| 270 mL 1x TAE + 5.4 g agarose |
2 µL gel load dye + 4 µL PCR product |
160 V
|
| 27 µL GelRed |
6 µL ladder |
45 minutes
|
- mispriming everywhere - primer pair not usable for CNHY
- lots of mispriming, but may be able to use conditions indicated by orange face (
 ), if needed, under stringent conditions
- favorable conditions indicated by
CNTI Cvanµ24
PCR
Gel
| Pour |
Load |
Run
|
| 270 mL 1x TAE + 5.4 g agarose |
2 µL gel load dye + 4 µL PCR product |
160 V
|
| 27 µL GelRed |
6 µL ladder |
45 minutes
|
- mispriming everywhere - primer pair not usable for CNHY
- lots of mispriming, but may be able to use conditions indicated by orange face (), if needed, under stringent conditions
- favorable conditions indicated by
CNTI Cvanµ26
PCR
Gel
| Pour |
Load |
Run
|
| 270 mL 1x TAE + 5.4 g agarose |
2 µL gel load dye + 4 µL PCR product |
160 V
|
| 27 µL GelRed |
6 µL ladder |
45 minutes
|
- mispriming everywhere - primer pair not usable for CNHY
- lots of mispriming, but may be able to use conditions indicated by orange face (), if needed, under stringent conditions
- favorable conditions indicated by
CNTI Cvanµ27
PCR
Gel
| Pour |
Load |
Run
|
| 270 mL 1x TAE + 5.4 g agarose |
2 µL gel load dye + 4 µL PCR product |
160 V
|
| 27 µL GelRed |
6 µL ladder |
45 minutes
|
- mispriming everywhere - primer pair not usable for CNHY
- lots of mispriming, but may be able to use conditions indicated by orange face (), if needed, under stringent conditions
- favorable conditions indicated by
|
Tara's Lab Notebook
