User:Tara K. Luckau/Notebook/Team ConGen/2013/02/09

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Current revision (17:43, 12 February 2013) (view source)
 
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==CNTI Cvanµ23==
+
==ASHY Cvanµ23==
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[[Image:20130209_Gela.png|800 px]]
[[Image:20130209_Gela.png|800 px]]
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* mispriming everywhere - primer pair not usable for CNHY
+
* mispriming everywhere - primer pair not usable for ASHY
-
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
+
-
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
+
-
==CNTI Cvanµ24==
+
==ASHY Cvanµ24==
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[[Image:20130209_Gelb.png|800 px]]
[[Image:20130209_Gelb.png|800 px]]
-
* mispriming everywhere - primer pair not usable for CNHY
+
* it works! Yay! Many possibilities
-
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
+
-
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
+
-
==CNTI Cvanµ26==
+
==ASHY Cvanµ26==
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[[Image:20130209_Gelc.png|800 px]]
[[Image:20130209_Gelc.png|800 px]]
-
* mispriming everywhere - primer pair not usable for CNHY
+
* mispriming everywhere - primer pair not usable for ASHY
-
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
+
-
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
+
-
==CNTI Cvanµ27==
+
==ASHY Cvanµ27==
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[[Image:20130209_Geld.png|800 px]]
[[Image:20130209_Geld.png|800 px]]
-
* mispriming everywhere - primer pair not usable for CNHY
+
* mispriming everywhere - primer pair not usable for ASHY
-
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
+
-
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
+

Current revision

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Contents


ASHY Cvanµ23

Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


  • mispriming everywhere - primer pair not usable for ASHY


ASHY Cvanµ24

PCR


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


  • it works! Yay! Many possibilities


ASHY Cvanµ26

PCR


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


  • mispriming everywhere - primer pair not usable for ASHY


ASHY Cvanµ27

PCR


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


  • mispriming everywhere - primer pair not usable for ASHY




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