User:Tara K. Luckau/Notebook/Team ConGen/2013/02/09

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(Autocreate 2013/02/09 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
Current revision (16:43, 12 February 2013) (view source)
 
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==CNTI Acos5==
+
==ASHY Cvanµ23==
 +
 
 +
 
 +
===Gel===
 +
 
 +
{| {{table}} style="text-align: center"
 +
|-
 +
! Pour !! Load !! Run
 +
|-
 +
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
 +
|-
 +
| 27 µL GelRed || 6 µL ladder || 45 minutes
 +
|}
 +
 
 +
 
 +
[[Image:20130209_Gela.png|800 px]]
 +
 
 +
* mispriming everywhere - primer pair not usable for ASHY
 +
 
 +
 
 +
==ASHY Cvanµ24==
 +
 
 +
 
 +
===PCR===
 +
* [[Image:20130209_PCRa.png|800 px]]
 +
 
 +
 
 +
===Gel===
 +
 
 +
{| {{table}} style="text-align: center"
 +
|-
 +
! Pour !! Load !! Run
 +
|-
 +
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
 +
|-
 +
| 27 µL GelRed || 6 µL ladder || 45 minutes
 +
|}
 +
 
 +
 
 +
[[Image:20130209_Gelb.png|800 px]]
 +
 
 +
* it works! Yay! Many possibilities
 +
 
 +
 
 +
==ASHY Cvanµ26==
 +
 
 +
 
 +
===PCR===
 +
* [[Image:20130209_PCRb.png|800 px]]
 +
 
 +
 
 +
===Gel===
 +
 
 +
{| {{table}} style="text-align: center"
 +
|-
 +
! Pour !! Load !! Run
 +
|-
 +
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
 +
|-
 +
| 27 µL GelRed || 6 µL ladder || 45 minutes
 +
|}
 +
 
 +
 
 +
[[Image:20130209_Gelc.png|800 px]]
 +
 
 +
* mispriming everywhere - primer pair not usable for ASHY
 +
 
 +
 
 +
==ASHY Cvanµ27==
===PCR===
===PCR===
-
* [[Image:20120110_PCRa.png|800 px]]
+
* [[Image:20130209_PCRc.png|800 px]]
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[[Image:20120110_Gela.png|800 px]]
+
[[Image:20130209_Geld.png|800 px]]
-
* mispriming everywhere - primer pair not usable for CNHY
+
* mispriming everywhere - primer pair not usable for ASHY
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* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
+
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* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
+

Current revision

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Contents


ASHY Cvanµ23

Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


  • mispriming everywhere - primer pair not usable for ASHY


ASHY Cvanµ24

PCR


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


  • it works! Yay! Many possibilities


ASHY Cvanµ26

PCR


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


  • mispriming everywhere - primer pair not usable for ASHY


ASHY Cvanµ27

PCR


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


  • mispriming everywhere - primer pair not usable for ASHY




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