User:Tara K. Luckau/Notebook/Team ConGen/2013/02/08: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2013/02/08 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
 
No edit summary
(One intermediate revision by the same user not shown)
Line 9: Line 9:




==CNTI Acos5==
==ASHY Cvanµ18==
 
 
===Gel===
 
{| {{table}} style="text-align: center"
|-
! Pour !! Load !! Run
|-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
|-
| 27 µL GelRed || 6 µL ladder || 45 minutes
|}
 
 
[[Image:20130208_Gela.png|800 px]]
 
* mispriming everywhere - primer pair not usable for ASHY
 
 
==ASHY Cvanµ19==




===PCR===
===PCR===
* [[Image:20120110_PCRa.png|800 px]]
* [[Image:20130208_PCRa.png|800 px]]




Line 28: Line 48:




[[Image:20120110_Gela.png|800 px]]
[[Image:20130208_Gelb.png|800 px]]


* mispriming everywhere - primer pair not usable for CNHY
* no amplification - primer pair not usable for ASHY
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
 
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
 
==ASHY Cvanµ23==
 
 
===PCR===
* [[Image:20130208_PCRb.png|800 px]]




Revision as of 14:39, 12 February 2013

Tara's Lab Notebook <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


ASHY Cvanµ18

Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


  • mispriming everywhere - primer pair not usable for ASHY


ASHY Cvanµ19

PCR


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


  • no amplification - primer pair not usable for ASHY


ASHY Cvanµ23

PCR




Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Tara_K._Luckau/Notebook/Team_ConGen/2013/02/08&oldid=675850"