User:Tara K. Luckau/Notebook/Team ConGen/2013/02/08: Difference between revisions

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==CNTI Cvanµ18==
==ASHY Cvanµ18==




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[[Image:20130208_Gela.png|800 px]]
[[Image:20130208_Gela.png|800 px]]


* mispriming everywhere - primer pair not usable for CNHY
* mispriming everywhere - primer pair not usable for ASHY
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]




==CNTI Cvanµ19==
==ASHY Cvanµ19==




Line 52: Line 50:
[[Image:20130208_Gelb.png|800 px]]
[[Image:20130208_Gelb.png|800 px]]


* mispriming everywhere - primer pair not usable for CNHY
* no amplification - primer pair not usable for ASHY
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]




==CNTI Cvanµ23==
==ASHY Cvanµ23==




===PCR===
===PCR===
* [[Image:20130208_PCRb.png|800 px]]
* [[Image:20130208_PCRb.png|800 px]]





Revision as of 14:39, 12 February 2013

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ASHY Cvanµ18

Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


  • mispriming everywhere - primer pair not usable for ASHY


ASHY Cvanµ19

PCR


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


  • no amplification - primer pair not usable for ASHY


ASHY Cvanµ23

PCR