User:Tara K. Luckau/Notebook/Team ConGen/2013/02/08

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Current revision (17:39, 12 February 2013) (view source)
 
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==CNTI Cvanµ18==
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==ASHY Cvanµ18==
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[[Image:20130208_Gela.png|800 px]]
[[Image:20130208_Gela.png|800 px]]
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* mispriming everywhere - primer pair not usable for CNHY
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* mispriming everywhere - primer pair not usable for ASHY
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* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
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* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
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==CNTI Cvanµ19==
+
==ASHY Cvanµ19==
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[[Image:20130208_Gelb.png|800 px]]
[[Image:20130208_Gelb.png|800 px]]
-
* mispriming everywhere - primer pair not usable for CNHY
+
* no amplification - primer pair not usable for ASHY
-
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
+
-
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
+
-
==CNTI Cvanµ23==
+
==ASHY Cvanµ23==
===PCR===
===PCR===
* [[Image:20130208_PCRb.png|800 px]]
* [[Image:20130208_PCRb.png|800 px]]
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Current revision

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Contents


ASHY Cvanµ18

Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


  • mispriming everywhere - primer pair not usable for ASHY


ASHY Cvanµ19

PCR


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


  • no amplification - primer pair not usable for ASHY


ASHY Cvanµ23

PCR




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