User:Tara K. Luckau/Notebook/Team ConGen/2013/02/07

Current revision

Tara's Lab Notebook

Pour	Load	Run
270 mL 1x TAE + 5.4 g agarose	2 µL gel load dye + 4 µL PCR product	160 V
27 µL GelRed	6 µL ladder	45 minutes

Pour	Load	Run
270 mL 1x TAE + 5.4 g agarose	2 µL gel load dye + 4 µL PCR product	160 V
27 µL GelRed	6 µL ladder	45 minutes

@@ Line 9: / Line 9: @@
-==CNTI Acos5==
+==ASHY Cvanµ4==
 ===PCR===
-* [[Image:20120110_PCRa.png|800 px]]
+* [[Image:20130207_PCRa.png|800 px]]
@@ Line 28: / Line 28: @@
-[[Image:20120110_Gela.png|800 px]]
+[[Image:20130207_Gela.png|800 px]]
-* mispriming everywhere - primer pair not usable for CNHY
+* mispriming everywhere - primer pair not usable for ASHY
-* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
-* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
+==ASHY Cvanµ7==
+===PCR===
+* [[Image:20130207_PCRb.png|800 px]]
+===Gel===
+{| {{table}} style="text-align: center"
+|-
+! Pour !! Load !! Run
+|-
+| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
+|-
+| 27 µL GelRed || 6 µL ladder || 45 minutes
+|}
+[[Image:20130207_Gelb.png|800 px]]
+* mispriming everywhere - primer pair not usable for ASHY
+==ASHY Cvanµ18==
+===PCR===
+* [[Image:20130207_PCRc.png|800 px]]