User:Tara K. Luckau/Notebook/Team ConGen/2013/02/07

(Difference between revisions)

Revision as of 17:10, 12 February 2013

Tara's Lab Notebook

Pour	Load	Run
270 mL 1x TAE + 5.4 g agarose	2 µL gel load dye + 4 µL PCR product	160 V
27 µL GelRed	6 µL ladder	45 minutes

mispriming everywhere - primer pair not usable for CNHY
lots of mispriming, but may be able to use conditions indicated by orange face (), if needed, under stringent conditions
favorable conditions indicated by

Pour	Load	Run
270 mL 1x TAE + 5.4 g agarose	2 µL gel load dye + 4 µL PCR product	160 V
27 µL GelRed	6 µL ladder	45 minutes

mispriming everywhere - primer pair not usable for CNHY
lots of mispriming, but may be able to use conditions indicated by orange face (), if needed, under stringent conditions
favorable conditions indicated by

@@ Line 9: / Line 9: @@
-==CNTI Acos5==
+==CNTI Cvanµ4==
 ===PCR===
-* [[Image:20120110_PCRa.png|800 px]]
+* [[Image:20130207_PCRa.png|800 px]]
@@ Line 28: / Line 28: @@
-[[Image:20120110_Gela.png|800 px]]
+[[Image:20130207_Gela.png|800 px]]
 * mispriming everywhere - primer pair not usable for CNHY
@@ Line 34: / Line 34: @@
 * favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
+==CNTI Cvanµ7==
+===PCR===
+* [[Image:20130207_PCRb.png|800 px]]
+===Gel===
+{| {{table}} style="text-align: center"
+|-
+! Pour !! Load !! Run
+|-
+| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
+|-
+| 27 µL GelRed || 6 µL ladder || 45 minutes
+|}
+[[Image:20130207_Gelb.png|800 px]]
+* mispriming everywhere - primer pair not usable for CNHY
+* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
+* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
+==CNTI Cvanµ18==
+===PCR===
+* [[Image:20130207_PCRc.png|800 px]]