User:Tara K. Luckau/Notebook/Team ConGen/2013/02/07

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Current revision (16:36, 12 February 2013) (view source)
 
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==CNTI Cvanµ4==
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==ASHY Cvanµ4==
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[[Image:20130207_Gela.png|800 px]]
[[Image:20130207_Gela.png|800 px]]
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* mispriming everywhere - primer pair not usable for CNHY
+
* mispriming everywhere - primer pair not usable for ASHY
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* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
+
-
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
+
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==CNTI Cvanµ7==
+
==ASHY Cvanµ7==
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[[Image:20130207_Gelb.png|800 px]]
[[Image:20130207_Gelb.png|800 px]]
-
* mispriming everywhere - primer pair not usable for CNHY
+
* mispriming everywhere - primer pair not usable for ASHY
-
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
+
-
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
+
-
==CNTI Cvanµ18==
+
==ASHY Cvanµ18==

Current revision

Tara's Lab Notebook Main project page
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Contents


ASHY Cvanµ4

PCR


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


  • mispriming everywhere - primer pair not usable for ASHY


ASHY Cvanµ7

PCR


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


  • mispriming everywhere - primer pair not usable for ASHY


ASHY Cvanµ18

PCR



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