CNHY MP1a/b Optimization
- optimize for marker peak height - vary each primer (based on results from 23 July 2012)
- CNHY MP1a: increase Ai5013, Ai5043, Ai5071
- CNHY MP1a: decrease Acos5
- CNHY MP1b: increase Ai5057, Ai5062
Frag Results
MP1a
Acos3
- 23 July 2012: no changes needed
- this time:
- still fine, though seems to decrease with increasing Acos5 as well as Ai5071
Acos5
- 23 July 2012: too high
- this time:
- 0.04 = too low
- 0.06 = inconclusive (failures)
- 0.08 = too high
Ai5013
- 23 July 2012: too low
- this time:
- second peak never got above 250, regardless of concentration
- however, in Acos5 assay, noticed second peak reached 400 intensity as Acos5 decreased
Ai5043
- 23 July 2012: did not amplify
- this time: did not amplify
- remove from multiplex
Ai5071
- 23 July 2012: too low
- this time:
- best at 0.24, 0.28, 0.32 (200-300 intensity only)
MP1b
Ai5033
- 23 July 2012: no changes needed
- this time:
- still fine (no amp for any markers in Ai5057 assay)
Ai5035
- 23 July 2012: no changes needed
- this time:
- a bit low in Ai5062 assay (no amp for any markers in Ai5057 assay)
Ai5037
- 23 July 2012: no changes needed
- this time:
- still fine (no amp for any markers in Ai5057 assay)
Ai5057
- 23 July 2012: did not amplify
- this time: did not amplify
- remove from multiplex
Ai5062
- 23 July 2012: too low
- this time:
- best at 0.48 (400 & 6000 intensity)
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