User:Tara K. Luckau/Notebook/Team ConGen/2012/11/27: Difference between revisions
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* UAGC submission# YFPQ | * UAGC submission# YFPQ | ||
====MP1a==== | |||
=====Acos3===== | |||
* 23 July 2012: no changes needed | |||
* this time: | |||
: still fine, though seems to decrease with increasing Acos5 as well as Ai5071 | |||
=====Acos5===== | |||
* 23 July 2012: too high | |||
* this time: | |||
: 0.04 = too low | |||
: 0.06 = inconclusive (failures) | |||
: 0.08 = too high | |||
=====Ai5013===== | |||
* 23 July 2012: too low | |||
* this time: | |||
: second peak never got above 250, regardless of concentration | |||
: however, in Acos5 assay, noticed second peak reached 400 intensity as Acos5 decreased | |||
=====Ai5043===== | |||
* 23 July 2012: did not amplify | |||
* this time: did not amplify | |||
: remove from multiplex | |||
=====Ai5071===== | |||
* 23 July 2012: too low | |||
* this time: | |||
: best at 0.24, 0.28, 0.32 (200-300 intensity only) | |||
====MP1b==== | |||
=====Ai5033===== | |||
* 23 July 2012: no changes needed | |||
* this time: | |||
: still fine (no amp for any markers in Ai5057 assay) | |||
=====Ai5035===== | |||
* 23 July 2012: no changes needed | |||
* this time: | |||
: a bit low in Ai5062 assay (no amp for any markers in Ai5057 assay) | |||
=====Ai5037===== | |||
* 23 July 2012: no changes needed | |||
* this time: | |||
: still fine (no amp for any markers in Ai5057 assay) | |||
=====Ai5057===== | |||
* 23 July 2012: did not amplify | |||
* this time: did not amplify | |||
: remove from multiplex | |||
=====Ai5062===== | |||
* 23 July 2012: too low | |||
* this time: | |||
: best at 0.48 (400 & 6000 intensity) | |||
Revision as of 15:49, 27 November 2012
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CNHY MP1a/b Optimization
Frag Results
MP1aAcos3
Acos5
Ai5013
Ai5043
Ai5071
MP1bAi5033
Ai5035
Ai5037
Ai5057
Ai5062
|