User:Tara K. Luckau/Notebook/Team ConGen/2012/11/27

CNHY MP1a/b Optimization

• optimize for marker peak height - vary each primer (based on results from 23 July 2012)
• CNHY MP1a: increase Ai5013, Ai5043, Ai5071
• CNHY MP1a: decrease Acos5
• CNHY MP1b: increase Ai5057, Ai5062

Frag Results

• UAGC submission# YFPQ

MP1a

Acos3

• 23 July 2012: no changes needed
• this time:

still fine, though seems to decrease with increasing Acos5 as well as Ai5071

Acos5

• 23 July 2012: too high
• this time:

0.04 = too low

0.06 = inconclusive (failures)

0.08 = too high

Ai5013

• 23 July 2012: too low
• this time:

second peak never got above 250, regardless of concentration

however, in Acos5 assay, noticed second peak reached 400 intensity as Acos5 decreased

Ai5043

• 23 July 2012: did not amplify
• this time: did not amplify

remove from multiplex

Ai5071

• 23 July 2012: too low
• this time:

best at 0.24, 0.28, 0.32 (200-300 intensity only)

MP1b

Ai5033

• 23 July 2012: no changes needed
• this time:

still fine (no amp for any markers in Ai5057 assay)

Ai5035

• 23 July 2012: no changes needed
• this time:

a bit low in Ai5062 assay (no amp for any markers in Ai5057 assay)

Ai5037

• 23 July 2012: no changes needed
• this time:

still fine (no amp for any markers in Ai5057 assay)

Ai5057

• 23 July 2012: did not amplify
• this time: did not amplify

remove from multiplex

Ai5062

• 23 July 2012: too low
• this time:

best at 0.48 (400 & 6000 intensity)